Morphological regulation of rabbit chondrocytes on glucose-displayed surface
Morphological regulation of rabbit chondrocytes on glucose-displayed surface
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DOI：
10.1016/j.biomaterials.2006.11.037
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Taya, Masahito
中科院分区：
文献类型：
--
作者：
Kino-oka, Masahiro;Morinaga, Yoshiki;Taya, Masahito
通过改变玻片上显示的D-和L-葡萄糖异构体的比例，设计了一种调节兔软骨细胞形态的培养表面。随着D-葡萄糖在细胞表面展示比例的增加，细胞的附着效率提高，这意味着细胞的附着只通过D-葡萄糖与细胞膜上的葡萄糖转运体之间的亲和力来实现。在0%和100%D-葡萄糖显示时，圆形细胞占主导地位，而在50%D-葡萄糖显示时，大部分细胞呈伸展状，显示圆形细胞的频率与显示的葡萄糖比率呈凹形。F-肌动蛋白和纽蛋白的细胞骨架染色显示，圆形细胞和100%D-葡萄糖显影表面呈伸展状的细胞均可见未成熟的应力纤维，局灶性接触较少。时间推移观察显示，细胞在100%D-葡萄糖显示表面进行了活跃的迁移和聚集，形成了II型胶原。这些结果表明，100%D-葡萄糖显示表面可以提供与三维培养相似的培养环境，以维持细胞的软骨细胞表型。(C)2006爱思唯尔有限公司。保留所有权利。
A culture surface was designed to regulate morphology of rabbit chondrocytes by changing the ratio of D- and L-glucose isomers displayed on a glass plate. With increasing ratio Of D-glucose displayed on the surfaces, the efficiency of cell attachment improved, meaning that the attachment exclusively occurred via mediation of an affinity between D-glucose displayed and glucose transporter on cell membrane. At 0% and 100% D-glucose display, the round-shaped cells appeared dominantly, and most of cells became stretched in shape at 50% D-glucose display, indicating that the frequency of round-shaped cells depicted a concave profile against the ratio of Dglucose displayed. From the cytoskeletal staining of F-actin and vinculin, the immature stress fibers with fewer focal contacts were recognized in both the round shaped cells and those stretched in shape on 100% D-glucose-displayed surface. The time-lapse observation revealed that the cells on 100% D-glucose-displayed surface conducted active migration and aggregation with formation of collagen type II. These results suggest that 100% D-glucose-displayed surface can offer culture environment to maintain the chondrocytic phenotype of cells, similarly to the conditions achieved in three-dimensional (3-D) culture. (c) 2006 Elsevier Ltd. All rights reserved.