MicroRNA-1 and MicroRNA-21 Individually Regulate Cellular Growth of Non-malignant and Malignant Renal Cells
MicroRNA-1 and MicroRNA-21 Individually Regulate Cellular Growth of Non-malignant and Malignant Renal Cells
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DOI：
10.21873/invivo.11103
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发表时间：
2017-07-01
期刊：
影响因子：
2.3
通讯作者：
Stope, Matthias B.
中科院分区：
文献类型：
--
作者：
Ahrend, Hannes;Kaul, Anne;Stope, Matthias B.
背景/目的：由于肾细胞癌（RCC）预后差，越来越有必要了解其生物学特性。因此，我们研究了microRNA miR-1和miR-21在RCC细胞生长中的作用，并与非恶性肾细胞进行了比较。材料与方法：检测了四种恶性细胞系（Caki-1、786-O、RCC 4、A498）的细胞生长、microRNA和端粒酶表达，并与非恶性RC-124肾细胞进行了比较。结果如下：在RCC细胞组中分别出现了关于miR-1和miR-21的抗增殖和增殖特性的抑制。值得注意的是，最有可能是由于永生化，非恶性RC-124细胞表现出端粒酶表达和活性。结论：miR-1和miR-21在癌症进展中的功能，特别是在肿瘤生长中，可能更依赖于个体细胞环境，并可能反映RCC异质性。因此，这两种microRNA与其他分层生物标志物结合，可能在RCC诊断、预后或治疗反应方面有用。
Background/Aim: Due to its poor prognosis, it is increasingly necessary to understand the biology of renal cell cancer (RCC). Therefore, we investigated the role of microRNAs miR-1 and miR-21 in the growth of RCC cells compared to that of non-malignant renal cells. Materials and Methods: Four malignant cell lines (Caki-1, 786-O, RCC4, A498) were examined regarding their cell growth, microRNA and telomerase expression, and were compared to nonmalignant RC-124 renal cells. Results: Inconsistencies appeared in the panel of RCC cells regarding antiproliferative and proliferative properties of miR-1 and miR-21, respectively. Notably, and most likely due to immortaliziation, nonmalignant RC-124 cells exhibited telomerase expression and activity. Conclusion: miR-1 and miR-21 functionality in cancer progression, particularly in tumor growth, may be more dependent on the individual cellular context and may reflect RCC heterogeneity. Thus, both microRNAs, in combination with other stratifying biomarkers, may be useful in terms of RCC diagnosis, prognosis, or treatment response.