Clinical role for a superantigen in Yersinia pseudotuberculosis infection
Clinical role for a superantigen in Yersinia pseudotuberculosis infection
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DOI：
10.1172/jci119349
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发表时间：
1997-04-15
影响因子：
15.9
通讯作者：
Takeda, T
中科院分区：
文献类型：
--
作者：
Abe, J;Onimaru, M;Takeda, T
假结核耶尔森氏菌是一种肠道病原体，在人类中引起各种临床症状。最近，我们报道了超抗原（Y。pseudotuberculosis-derived mitogen，YPM）的基因结构。为进一步研究YPM在Y.本研究检测了急性感染Y.假结核与健康对照组相比，33例患者中有20例（61%）的抗体滴度升高（P = 0.0001）。有全身症状如淋巴结病、一过性肾功能不全和关节炎的患者抗YPM抗体滴度显著高于仅有胃肠道症状的患者。与健康儿童相比，急性期患者中携带V β 3基因片段的T细胞显著增加（P = 0.009）。在疾病的恢复期，V β 3 T细胞的异常水平降低。此外，在肠系膜淋巴结中，与外周血相比，V β 3 T细胞水平升高，并且在1例患者中观察到含有V β 3元件的T细胞抗原受体β链连接区的序列多样性。总之，这些发现表明YPM是在体内产生的，并在Y.假结核感染
Yersinia pseudotuberculosis is an enteric pathogen that causes a variety of clinical symptoms in the human. Recently, we reported the production of a superantigen (Y. pseudotuberculosis-derived mitogen, YPM) by this organism and characterized the gene structure of ypm. To further study the potential pathogenic role of YPM in Y. pseudotuberculosis infection, we assayed IgG anti-YPM antibodies and T cell antigen receptor-V beta expression of the T cells in peripheral blood and in mesenteric lymph node in patients acutely infected with Y. pseudotuberculosis. 20 out of 33 patients (61%) had an elevated antibody titer compared with healthy controls (P = 0.0001). Patients with systemic symptoms such as lymphadenopathy, transient renal dysfunction, and arthritis had significantly higher titers of anti-YPM than patients with gastrointestinal tract symptoms alone. T cells bearing the V beta 3 gene segment were significantly increased (P = 0.009) among acute phase patients compared with healthy children. During the convalescence phase of the illness, there was a reduction in the abnormal level of V beta 3 T cells. Moreover, in the mesenteric lymph node, an elevated level of V beta 3 T cells compared with peripheral blood and a sequence diversity in the junctional region of the T cell antigen receptor beta-chain containing V beta 3 element was observed in one patient. Together, these findings suggest that YPM was produced in vivo and played an important role in the pathogenesis of Y. pseudotuberculosis infection.