Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates.
Genetic and phenotypic analysis of biofilm phenotypic variation in multiple Staphylococcus epidermidis isolates.
复制标题
多种表皮葡萄球菌分离株生物膜表型变异的遗传和表型分析。
DOI：
10.1099/jmm.0.05372-0
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发表时间：
2004
影响因子：
3
通讯作者：
O'Gara,JP
中科院分区：
文献类型：
--
作者：
Handke,LD;Conlon,KM;Slater,SR;Elbaruni,S;Fitzpatrick,F;Humphreys,H;Giles,WP;Rupp,ME;Fey,PD;O'Gara,JP
Production of biofilm inStaphylococcus epidermidisis mediated through enzymes produced by the four-gene operonicaand is subject to phenotypic variation. The purpose of these experiments was to investigate the regulation oficaandicaRtranscription in phenotypic variants produced by multiple unrelated isolates ofS. epidermidis. Ten isolates were chosen for the study, four of which contained IS256. IS256mediates a reversible inactivation oficain approximately 30 % of phenotypic variants. All ten strains produced at least two types of phenotypic variant (intermediate and smooth) in which biofilm formation was significantly impaired. Reversion studies indicated that all phenotypic variants were stable after overnight growth, but began to revert to other phenotypic forms after 5 days of incubation at 37 °C.icatranscriptional analysis was performed on phenotypic variants from three IS256-negative isolates; 1457, SE5 and 14765. This analysis demonstrated thaticatranscription was significantly reduced in the majority of phenotypic variants, although two variants from SE5 and 1457 produced wild-type quantities oficatranscript. Analysis of seven additional phenotypic variants from SE5 revealed thaticaexpression was only reduced in three. Expression oficaRtranscript was unaffected in all smooth phenotypic variants. Mutations withinicawere identified in two SE5 variants with wild-type levels oficatranscription. It is concluded that mutation and transcriptional regulation oficaare the primary mechanisms that govern phenotypic variation of biofilm formation within IS256-negativeS. epidermidis.
