DNA sequence analysis of a mouse pro alpha 1 (I) procollagen gene: evidence for a mouse B1 element within the gene
DNA sequence analysis of a mouse pro alpha 1 (I) procollagen gene: evidence for a mouse B1 element within the gene
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小鼠 pro alpha 1 (I) 前胶原基因的 DNA 序列分析：基因内存在小鼠 B1 元件的证据
DOI：
10.1128/mcb.2.11.1362-1371.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
B. Mccarthy
中科院分区：
文献类型：
--
作者：
J. M. Monson;J. Friedman;B. Mccarthy
在编码I型前胶原的前α 1（I）链的氨基酸序列的3.8-腺苷酸酶小鼠DNA序列中，鉴定了14个编码序列，其指定了与牛α 1（I）链的氨基酸残基568至963具有95%同源性的序列。所有这些编码序列的侧翼都是遵循GT/AG规则的适当剪接点。这些观察结果表明，但不能证明，这前α 1（I）基因是转录活性。在14个编码序列中，7个长度为54个碱基对，而其余的是54个碱基对的更高倍数。非随机利用的密码子percontained在整个所有的编码序列显示出偏好（56%）U的摆动位置。两个插入序列编码的编码序列的不完美痕迹，表现出密码子偏好不同的亲α 1（I）基因本身，并没有侧翼剪接点。一个插入序列编码小鼠B1家族的中间重复序列的成员。它的两侧是8个碱基对的直接重复序列，并有一个截短的A-丰富的区域，这表明它可能是一个移动的元件。在该元件内的序列可以作为RNA聚合酶III分裂启动子。
In a 3.8-kilobase mouse DNA sequence encoding amino acid sequences for the pro alpha 1(I) chain of type I procollagen, 14 coding sequences were identified which specify a sequence 95% homologous to amino acid residues 568 to 963 of the bovine alpha 1(I) chain. All of these coding sequences were flanked by appropriate splice junctions following the GT/AG rule. These observations suggest, but do not prove, that this pro alpha 1(I) gene is transcriptionally active. Of the 14 coding sequences, 7 were 54 base pairs in length, whereas the remainder were higher multiples of 54 base pairs. Nonrandom utilization of codons pertained throughout all of the coding sequences showing a preference (56%) for U in the wobble position. Two of the intervening sequences encoded imperfect vestiges of coding sequences which exhibited a codon preference different from that of the pro alpha 1(I) gene proper and were not flanked by splice junctions. One intervening sequence encoded a member of the mouse B1 family of middle repetitive sequences. It was flanked by 8-base-pair direct repeats and had a truncated A-rich region, suggesting that it may be a mobile element. Within this element were sequences which could function as a RNA polymerase III split promoter.
对散布在人类 β 样珠蛋白基因中的 Alu 家族序列的模板和 RNA 聚合酶 III 转录本进行结构分析。
影响因子：
3.5
作者：
Duncan,CH;Jagadeeswaran,P;Wang,RR;Weissman,SM
通讯作者：
Weissman,SM