NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
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DOI：
10.1016/0014-4827(68)90403-5
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发表时间：
1968-01-01
影响因子：
3.7
通讯作者：
OJIMA, K
中科院分区：
文献类型：
--
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
对大豆根段悬浮培养的营养需求进行了研究。L.），并开发了一种简单的培养基。培养基由矿物盐、蔗糖、B族维生素、m-肌醇和2，4-二氯苯氧基乙酸（2，4-D）组成。证明了对三胺和2，4-D的绝对需求。氮以硝酸盐和铵的形式提供。铵或其他还原氮源是生长所必需的。KNO 3和（NH 4）2SO 4的最佳浓度分别为25和1 mM。在初始pH为5.0时细胞产量最高。在生长周期中，pH逐渐增加至6.2。
Investi-gations were carried out on the nutrient requirements of suspension cultures derived from root sections of soybean (Glycine max. L.), and a simple medium was developed. The medium consists of mineral salts, sucrose, the B-vitamins, m-inositol and 2,4-dichlorophenoxy-acetic acid (2, 4-D). An absolute requirement for thlamine and 2, 4-D was demonstrated. N was supplied as nitrate and ammonium. Ammonium or some other source of reduced N was necessary for growth. Optimum concentration of KNO3 and (NH4)2SO4 were 25 and 1 mM, respectively. The highest yield of cells was achieved at an initial pH of 5.0. During the growth cycle the pH gradually increased to 6.2.