The preparation of gold nanoparticles and evaluation of their immunological function effects on rats.
The preparation of gold nanoparticles and evaluation of their immunological function effects on rats.
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DOI：
10.3233/bme-130882
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发表时间：
2014
影响因子：
1
通讯作者：
Yong-Tang Wang;Xiu-Min Lu;Feng Zhu;Min-Li Zhao
中科院分区：
文献类型：
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作者：
Yong-Tang Wang;Xiu-Min Lu;Feng Zhu;Min-Li Zhao
金纳米粒子（gold nanoparticles，GNP），又称胶体金（colloidal gold，CG），作为一种新型的生物材料，在生物医学领域有着广泛的应用。采用柠檬酸钠还原法制备了粒径分别为10、15和25 nm的GNP，并分别通过普通光学性质、紫外-可见（UV-vis）吸收光谱和扫描电镜（SEM）对其粒径和均匀性进行了表征。为了观察GNP对免疫功能的影响，用上述三种GNP免疫成年SD大鼠，每种剂量为0.2、0.4和0.6 ml，未免疫的大鼠作为阴性对照。免疫后，分别用噻唑蓝（MTT）法和酶联免疫吸附法（ELISA）检测血液和脾淋巴细胞的增殖活性以及血清和脾淋巴细胞培养上清中白细胞介素2（IL-2）的水平。结果表明，制备出了不同粒径的纳米颗粒，且随着粒径的减小，纳米颗粒的均匀性提高。不同粒径和剂量的纳米颗粒对小鼠血液和脾淋巴细胞增殖、血清和脾淋巴细胞培养上清中IL-2水平的影响不同。15 nm CG 0.6ml剂量组能显著促进血液和脾淋巴细胞增殖，提高血清和脾淋巴细胞培养上清中IL-2水平。结果表明，应用柠檬酸钠还原法制备的CG可增强特异性和非特异性免疫应答，其中0.6ml剂量的15 nm CG可能是最佳免疫剂量。这一事实可能作为进一步的证据，使用CG作为一种新型的免疫佐剂在未来。
As a new type of biomaterials, gold nanoparticles (GNPs), also known as colloidal gold (CG), have a wide biomedical application. In this study, GNPs with diameters of 10, 15, and 25 nm were prepared by sodium citrate reduction, and detected by common optical property, ultraviolet-visible (UV-vis) absorbance spectroscopy, and scanning electron microscope (SEM), separately for identification of the particle size and uniformity. In order to observe the effects of GNPs on immune function, adult Sprague Dawley (SD) rats were immunized with the above three GNPs, each having three doses of 0.2, 0.4, and 0.6 ml, and rats without immunization served as negative control. After immunization, proliferation activity of blood and spleen lymphocyte and the levels of interleukin-2 (IL-2) in serum and supernatant of spleen lymphocyte were detected by thiazoleblue (MTT) assay and enzyme linked immunosorbent assay (ELISA), respectively. The results indicated that different size of GNPs was prepared, and the uniformity increased with the decrease of the size of particles. Different diameters and doses of GNPs have different effects on proliferation of blood and spleen lymphocyte, as well as the levels of IL-2 in serum and supernatant of spleen lymphocyte. The 15 nm CG in 0.6 ml dose group could most significantly promote blood and spleen lymphocyte proliferation, and enhance IL-2 levels in serum and supernatant of spleen lymphocyte. Taken together, the findings revealed that application of CG prepared by sodium citrate reduction could enhance specific and nonspecific immune responses, and the 0.6 ml dose of 15 nm CG might be the best immunizing dose in rats. This fact may serve as a further evidence for using CG as a novel immunoadjuvant in the future.