PERMEABILIZATION OF PLANT-CELLS FOR RELEASE OF INTRACELLULARLY STORED PRODUCTS - VIABILITY STUDIES
PERMEABILIZATION OF PLANT-CELLS FOR RELEASE OF INTRACELLULARLY STORED PRODUCTS - VIABILITY STUDIES
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DOI：
10.1007/bf00451632
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发表时间：
1988-02-01
影响因子：
5
通讯作者：
BRODELIUS, P
中科院分区：
文献类型：
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作者：
BRODELIUS, P
研究了不同化学物质对三种悬浮培养物（Catharanthus roseus， Thalictrum rugosum和Chenopodium rubrum）质膜通透性和叶绿体通透性的影响。质膜的渗透性是通过测量胞质酶异柠檬酸脱氢酶的活性来监测的，而质体的渗透性是通过测定液泡中储存的物质的释放来测量的（三种细胞系分别为无机磷酸盐、小檗碱和甜菜素）。对五种不同的渗透剂进行了定量释放所需的最小浓度测定。除了DMSO和Triton X-100处理外，细胞活力在渗透后丧失。
The effects of various chemical substances on the permeability of plasma membranes and tonoplasts of three suspension cultures (Catharanthus roseus, Thalictrum rugosum and Chenopodium rubrum) have been studied. The permeability of the plasma membrane is monitored by measuring the activity of the cytosolic enzyme isocitrate dehydrogenase and the permeability of the tonoplast is measured by determining the release of substancec stored in the vacuoles (inorganic phosphate, berberine and betanin for the three cell lines, respectively). The minimum concentrationn required for quantitative release of vacuolar products have been established for five different permeabilization agents. Cell viability is lost upon permeabilization except for treatment of Catharanthus roseus with DMSO and Triton X-100.