TRANSFER OF GENE FOR THYMIDINE KINASE TO THYMIDINE KINASE-DEFICIENT HUMAN CELLS BY PURIFIED HERPES-SIMPLEX VIRAL-DNA
TRANSFER OF GENE FOR THYMIDINE KINASE TO THYMIDINE KINASE-DEFICIENT HUMAN CELLS BY PURIFIED HERPES-SIMPLEX VIRAL-DNA
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DOI：
10.1073/pnas.74.4.1590
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
GRAHAM, FL
中科院分区：
文献类型：
--
作者：
BACCHETTI, S;GRAHAM, FL
使用来自 2 型单纯疱疹病毒的纯化 DNA，将人类细胞从胸苷激酶（ATP：胸苷 5''-磷酸转移酶，EC 2.7.1.75）阴性转化为胸苷激酶阳性表型。DNA 的比活性范围为 0.5-2.0 个转化体/μg，基因转移效率高达 1 个转化体/105 个受体细胞。建立了几种能够在胸苷激酶阳性细胞选择性培养基中连续生长的转化系。所有这些细胞系都表达病毒来源的胸苷激酶活性，但它们在酶表达的稳定性方面彼此不同。来自给定转化系的亚克隆继承了亲本系的稳定性程度。
Transformation of human cells from a thymidine kinase (ATP:thymidine 5''-phosphotransferase, EC 2.7.1.75)-negative to a thymidine kinase-positive phenotype was achieved by using purified DNA from herpes simplex virus type 2. The specific activity of the DNA was in the range 0.5-2.0 transformants/.mu.g and the gene transfer efficiency was up to 1 transformant/105 recipient cells. Several transformed lines able to grow continuously in medium selective for thymidine kinase-positive cells were established. All of these lines express a thymidine kinase activity of viral origin but they differ from each other in the stability of enzyme expression. Subclones derived from a given transformed line inherited the degree of stability of the parental line.