Acquisition of iron by Gardnerella vaginalis
Acquisition of iron by Gardnerella vaginalis
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DOI：
10.1128/iai.66.10.5041-5047.1998
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发表时间：
1998-10-01
影响因子：
3.1
通讯作者：
Mercer, T
中科院分区：
文献类型：
--
作者：
Jarosik, GP;Land, CB;Mercer, T
对6株阴道加德纳氏菌利用各种含铁化合物作为铁源的能力进行了检测。在平板生物测定中，所有六个菌株都从氯化亚铁、三氯化铁、硫酸亚铁、柠檬酸铁铵、硫酸亚铁铵、牛和马的氯化血红素、牛的过氧化氢酶以及马、牛、兔和人的血红蛋白中获得铁。所有六个菌株也从人乳铁蛋白中获得铁，但通过液体肉汤生长试验确定不是从人转铁蛋白中获得铁。用铬天青S通用化学分析法检测了8株阴道毛滴虫的铁载体的产生。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法对富铁和限铁条件下培养的阴道毛滴虫594细胞质膜蛋白进行了分析，发现了几种铁调节蛋白，分子量在33~94 kDa之间。这些结果表明，阴道毛滴虫可能从铁盐和宿主铁化合物中获取铁。
Six Gardnerella vaginalis strains were examined for the ability to utilize various iron-containing compounds as iron sources. In a plate bioassay, all six strains acquired iron from ferrous chloride, ferric chloride, ferrous sulfate, ferric ammonium citrate, ferrous ammonium sulfate, bovine and equine hemin, bovine catalase, and equine, bovine, rabbit, and human hemoglobin. All six strains also acquired iron from human lactoferrin, but not from human transferrin, as determined by a liquid broth growth assay. Siderophore production was detected in eight G. vaginalis strains by the chrome azurol S universal chemical assay. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the cytoplasmic membrane proteins isolated from G. vaginalis 594 grown under iron-replete and iron-restricted conditions revealed several iron-regulated proteins ranging in molecular mass from 33 to 94 kDa. These results indicate that G. vaginalis may acquire iron from iron salts and host iron compounds.