A complex receptor locus confers responsiveness to necrosis and ethylene-inducing like peptides in Brassica napus
A complex receptor locus confers responsiveness to necrosis and ethylene-inducing like peptides in Brassica napus
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DOI：
10.1111/tpj.16760
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发表时间：
2024-04-11
期刊：
影响因子：
7.2
通讯作者：
Wells，Rachel
中科院分区：
文献类型：
--
作者：
Yalcin，Hicret Asli;Jacott，Catherine N.;Wells，Rachel
芸苔属作物易受病害的影响，而通过抗病育种可以减轻病害。MAMPs（微生物相关分子模式）是病原体的保守分子，可引发称为模式触发免疫（PTI）的宿主防御。坏死和乙烯诱导肽1样蛋白（NLP）是在广泛的植物病原体中发现的MAMP。我们研究了BcNEP 2对甘蓝型油菜抗病性的影响。为了绘制赋予NLP反应的区域，我们使用了在PTI期间在不同的B群体中诱导的活性氧（ROS）的产生。在计算机作图中，AT在染色体A04和C 05上鉴定了NLP响应性的两个峰，而BSA在A04上鉴定了一个峰。BSA界定了对3 Mbp的NLP响应性的区域，在Darmor-bzhreference基因组上含有约245个基因，并鉴定了4个共分离的KASP标记。与1911基因组相同的管道证实了染色体A04上的最高相关区域。比较BLAST分析显示，在B11染色体A04上存在未注释的受体样蛋白（RLP）同源物簇。然而，无法鉴定出赋予NLP反应的特定RLP同源物。我们的研究结果还表明BR信号激酶1可能参与调节NLP反应。总之，我们证明了对NLP的反应有助于B的抗病性。napusand定义相关的基因组位置。这些结果可在作物改良中得到实际应用。
Brassica crops are susceptible to diseases which can be mitigated by breeding for resistance. MAMPs (microbe‐associated molecular patterns) are conserved molecules of pathogens that elicit host defences known as pattern‐triggered immunity (PTI). Necrosis and Ethylene‐inducing peptide 1‐like proteins (NLPs) are MAMPs found in a wide range of phytopathogens. We studied the response to BcNEP2, a representative NLP fromBotrytis cinerea, and showed that it contributes to disease resistance inBrassica napus. To map regions conferring NLP response, we used the production of reactive oxygen species (ROS) induced during PTI across a population of diverseB. napusaccessions for associative transcriptomics (AT), and bulk segregant analysis (BSA) on DNA pools created from a cross of NLP‐responsive and non‐responsive lines.In silicomapping with AT identified two peaks for NLP responsiveness on chromosomes A04 and C05 whereas the BSA identified one peak on A04. BSA delimited the region for NLP‐responsiveness to 3 Mbp, containing ~245 genes on the Darmor‐bzhreference genome and four co‐segregating KASP markers were identified. The same pipeline with the ZS11 genome confirmed the highest‐associated region on chromosome A04. Comparative BLAST analysis revealed unannotated clusters of receptor‐like protein (RLP) homologues on ZS11 chromosome A04. However, no specific RLP homologue conferring NLP response could be identified. Our results also suggest thatBR‐SIGNALLING KINASE1may be involved with modulating the NLP response. Overall, we demonstrate that responsiveness to NLP contributes to disease resistance inB. napusand define the associated genomic location. These results can have practical application in crop improvement.