A human opsin-related gene that encodes a retinaldehyde-binding protein.
A human opsin-related gene that encodes a retinaldehyde-binding protein.
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编码视黄醛结合蛋白的人类视蛋白相关基因。
DOI：
10.1021/bi00248a022
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Fong,HK
中科院分区：
文献类型：
--
作者：
Shen,D;Jiang,M;Hao,W;Tao,L;Salazar,M;Fong,HK
材料和方法材料。人基因组DNA克隆从AFIX II噬菌体文库（Stratagene，Inc.，拉霍亚，CA），其通过将部分消化的扫罗A1 DNA片段插入XhoI克隆位点从人胎盘DNA构建。人视网膜cDNA文库由Jeremy Nathans（Johns霍普金斯大学医学院，巴尔的摩，MD）友好提供。使用Applied Biosystem 380 B DNA合成仪（Applied Biosystem，Foster City，
MATERIALS AND METHODSMaterials. Human genomic DNA clones were isolated from a AFIXII bacteriophage library (Stratagene, Inc., La Jolla, CA), which was constructed from human placental DNA by the insertion of partially digested Saul Al DNA fragments into the Xhol cloning site. The human retinal cDNA library was kindly provided by Jeremy Nathans (Johns Hopkins University School of Medicine, Baltimore, MD). Oligonucleotides were synthesized using an Applied Biosystems 380B DNA synthesizer (Applied Biosystems, Foster City,