Direct conjugation of semiconductor nanocrystals to a globular protein to study protein-folding intermediates
Direct conjugation of semiconductor nanocrystals to a globular protein to study protein-folding intermediates
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DOI：
10.1021/jp075239h
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发表时间：
2007-10-25
影响因子：
3.3
通讯作者：
Pal, Samir Kumar
中科院分区：
文献类型：
--
作者：
Sarkar, Rupa;Narayanan, S. Shankara;Pal, Samir Kumar
In this Article, we study the development of semiconductor nanocrystals (quantum dots of average diameter less than 2 nm) directly conjugated to a transporter protein human serum albumin (HSA) as fluorescent biological labels. Forster resonance energy transfer (FRET) from the amino acid tryptophan (Trp214) to quantum dot in HSA is monitored to follow the local and global changes in the protein structure during thermal unfolding and refolding processes. This study is likely to attract widespread attention as a powerful tool for the study of protein folding.
