Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells
Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells
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DOI：
10.1016/j.febslet.2008.10.023
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发表时间：
2008-11-12
期刊：
影响因子：
3.5
通讯作者：
Fukui, Kiichi
中科院分区：
文献类型：
--
作者：
Amin, Mohammed Abdullahel;Matsunaga, Sachihiro;Fukui, Kiichi
核磷蛋白（Nucleophosmin，NPM）是一种高表达的多功能核仁磷蛋白。在这里，我们表明，RNA干扰的NPM的耗尽导致细胞分裂缺陷，随后由于HeLa细胞中p53依赖性检查点反应的激活而导致DNA合成的停滞。由于纺锤体检查点激活，NPM的耗尽导致有丝分裂停滞。NPM缺失后的有丝分裂细胞在染色体聚集、纺锤体和中心体形成以及微管结合等方面都有缺陷。因此，NPM的丧失导致严重的有丝分裂缺陷和延迟的有丝分裂进展。这些发现表明，NPM是必不可少的有丝分裂的进展和细胞增殖。(C)2008年由Elsevier B出版。V.代表欧洲生物化学学会联合会。
Nucleophosmin (NPM) is an abundantly expressed multifunctional nucleolar phosphoprotein. Here we show that depletion of NPM by RNA interference causes defects in cell division, followed by an arrest of DNA synthesis due to activation of a p53-dependent checkpoint response in HeLa cells. Depletion of NPM leads to mitotic arrest due to spindle checkpoint activation. The mitotic cells arrested by NPM depletion have defects in chromosome congression, proper mitotic spindle and centrosome formation, as well as defects in kinetochoremicrotubule attachments. Loss of NPM thus causes severe mitotic defects and delayed mitotic progression. These findings indicate that NPM is essential for mitotic progression and cell proliferation. (C) 2008 Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies.