Spontaneous hybridization in the laboratory and genetic markers for the identification of hybrids between two atherinid species, Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909)
Spontaneous hybridization in the laboratory and genetic markers for the identification of hybrids between two atherinid species, Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909)
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DOI：
10.1111/j.1365-2109.1997.tb01045.x
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发表时间：
1997-04-01
影响因子：
2
通讯作者：
Takashima, F
中科院分区：
文献类型：
--
作者：
Strussmann, CA;Akaba, T;Takashima, F
本研究报道了博纳齿鱼（Odontesthes bonariensis 1835）与巴塔哥尼亚鱼（Patagonina hatcheri）之间的自发性（Eigenmann，1909），两种南美洲淡水动脉粥样硬化，而这两个物种的成年人被关押在一个公共实验室坦克，并描述了合适的遗传标记，以区分这两个物种及其杂交，对公共水箱中三次产卵的后代进行等位酶和线粒体DNA限制性长度多态性分析，证实了每次产卵中都存在杂交种沿着纯种，O的杂交种。bonariensis mother × a P，hatcheri father是沿着纯种O，bonariensis而产生的，而P，hatcheri mother × O.在两种情况下，沿着纯种哈氏原螯虾产生bonariensis父本，三种酶系统在15个（20个）位点中，用LDH-B*、PGM* 和CK* 位点进行了筛选，结果表明，LDH-B* 位点可区分种与种间杂种，但其在野外工作中的应用可能受到种内多态性的限制。（阿帕I、Bgl II、Eco RI、Eco RV、Eco T22 I、Hinc II、Hind III和Pvn II），并对这两个物种的自然遗传资源管理和水产养殖进行了讨论。
This study reports the occurrence of spontaneous between Odontesthes bonariensis 1835) and Patagonina hatcheri (Eigenmann, 1909), two South American freshwater atherinidsl while adults of both species were being held in a communal laboratory tank, and describes suitable genetic markers for distinction of both species and their hybrids, Combined allozyme and mitochondrial DNA restriction length polymorphism analyses of offspring from three spawnings in the communal tanks confirmed the presence of hybrids along with purebreds in each spawning, Hybrids of an O. bonariensis mother x a P, hatcheri father were produced along purebred O, bonariensis in one occasion, whereas hybrids of P, hatcheri mothers x O. bonariensis fathers were produced along purebred P. hatcheri in two occasions, Three enzyme systems (coded by loci LDH-B*, PGM*, and CK*) out of 15 (20 loci) screened allowed distinction of species and their hybrids, but the usefulness of LDH-B* for field work may be limited by intraspecific polymorphism, Eight restriction enzymes (Apa I, Bgl II, Eco RI, Eco RV,Eco T22I, Hinc II, Hind III, and Pvn II) among 11 produced diagnostic restriction fragments applicable for species (motherhood) distinction, The relevance of these findings for the management of natural genetic resources and the aquaculture of these two species is discussed.