NUCLEOTIDE-SEQUENCE OF THE SIMIAN SARCOMA-VIRUS GENOME - DEMONSTRATION THAT ITS ACQUIRED CELLULAR SEQUENCES ENCODE THE TRANSFORMING GENE-PRODUCT P28SIS
NUCLEOTIDE-SEQUENCE OF THE SIMIAN SARCOMA-VIRUS GENOME - DEMONSTRATION THAT ITS ACQUIRED CELLULAR SEQUENCES ENCODE THE TRANSFORMING GENE-PRODUCT P28SIS
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DOI：
10.1073/pnas.80.3.731
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
AARONSON, SA
中科院分区：
文献类型：
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作者：
DEVARE, SG;REDDY, EP;AARONSON, SA
猴肉瘤病毒（SSV）是一种灵长类动物来源的急性转化型逆转录病毒，其前病毒基因组的全核苷酸序列已测定。与其他转化病毒一样，SSV含有来自其辅助病毒（猿肉瘤相关病毒（SSAV））的序列和细胞衍生（v-sis）插入序列。通过与Moloney鼠白血病病毒的序列比较，可以精确定位和确定SSAV在SSV产生过程中所贡献的序列。对SSV-SSAV和SSV-SSAV的序列进行比较分析表明，SSAV提供了启动和终止SSV-SSAV转化基因转录的调控序列。该基因的推定蛋白产物的编码序列似乎起始于SSAV env基因的氨基末端。由用v-sis鉴定的开放阅读框预测的推定蛋白的羧基和氨基末端衍生的合成肽的抗体特异性地检测在SSV转化的大鼠肾细胞中的MW 28，000蛋白，p28 sis。这些和其他发现证实了该蛋白质的预测氨基酸序列，并将其定位于SSV转化基因的编码区。
The complete nucleotide sequence of the proviral genome of simian sarcoma virus (SSV), an acute transforming retrovirus of primate origin, was determined. Like other transforming viruses, SSV contains sequences derived from its helper virus, simian sarcoma-associated virus (SSAV), and a cell-derived (v-sis) insertion sequence. By comparison with the sequence of Moloney murine leukemia virus, it was possible to precisely localize and define sequences contributed by SSAV during the generation of SSV. Comparative sequence analysis of SSV and SSAV showed that SSAV provides regulatory sequences for initiation and termination of transcriptions of the SSV transforming gene. Coding sequences for the putative protein product of this gene appear to initiate from the amino terminus of the SSAV env gene. Antibodies to synthetic peptides derived from the carboxy and amino termini of the putative protein predicted by the open reading frame identified with v-sis specifically detect a MW 28,000 protein, p28sis, in SSV-transformed rat kidney cells. These and other findings confirm the predicted amino acid sequence of this protein and localize it to the coding region of the SSV transforming gene.