Comparative genomic analysis and evolution of family-B G protein-coupled receptors from six model insect species
Comparative genomic analysis and evolution of family-B G protein-coupled receptors from six model insect species
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六种模式昆虫 B 家族 G 蛋白偶联受体的比较基因组分析和进化
DOI：
10.1016/j.gene.2013.01.061
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发表时间：
2013-04-25
期刊：
影响因子：
3.5
通讯作者：
Li, Bin
中科院分区：
文献类型：
--
作者：
Li, Chengjun;Chen, Mei;Li, Bin
家族G蛋白偶联受体（gpcr - b）在包括生长、发育和生殖在内的许多生物过程中发挥着重要作用。然而，对昆虫中gpcr - b的进化和功能了解甚少。共从6种模式昆虫中鉴定出87种gpcr - b，其中，蜜蜂20种，蜜蜂9种，家蚕11种，沙蚕9种，冈比亚按蚊14种，黑腹果蝇24种。其中22例为本研究首次报道。系统发育分析表明，在昆虫进化过程中，gpcr - b发生了三种进化模式：一对一的同源关系、物种特异性扩增和某些昆虫谱系的偶发复制或丢失。一个引人注目的发现是在无脊椎动物中发现了甲状旁腺激素受体样基因（pthrl），该基因在脊椎动物和无脊椎动物中独立复制，而该基因在昆虫进化过程中至少丢失了两次。这些结果表明，PTHRL在哺乳动物和昆虫之间可能具有不同的功能。建立了非果蝇昆虫b家族gpcr的信息，对进一步研究这些gpcr的功能和去孤儿化具有重要意义。另一方面，本研究为我们提供了gpcr - b在差异生物中的多种功能，这也将成为新的农药和药物的潜在攻击靶点。(c) 2013 Elsevier B.V.版权所有
Family-B G protein-coupled receptors (GPCR-Bs) play vital roles in many biological processes, including growth, development and reproduction. However, the evolution and function of GPCR-Bs have been poorly understood in insects.We have identified 87 GPCR-Bs from six model insect species, 20 from Tribolium castaneum, 9 from Apis mellifera, 11 from Bombyx mori, 9 from Acyrthosiphon pisum, 14 from Anopheles gambiae and 24 from Drosophila melanogaster. 22 of them were reported in this study for the first time. Phylogenetic analysis revealed that there are three kinds of evolutionary patterns that occurred among GPCR-Bs during insect evolution: one-to-one orthologous relationships, species-specific expansion and episodic duplication or loss in certain insect lineages. A striking finding was the discovery of a parathyroid hormone receptor like gene (pthrl) in invertebrates, which was independently duplicated in vertebrates and invertebrates, whereas this gene was lost at least twice during insect evolution. These results indicate that PTHRL is possibly divergent in the functions between mammals and insects.The information of family-B GPCRs in nondrosophiline insects has been established, and will promote the further study on the function of these GPCRs and deorphanization of them. On the other hand, this study provides us with multiple function of GPCR-Bs in differential organisms, which will be also the potential attacking targets for new pesticides and drugs. (c) 2013 Elsevier B.V. All rights reserved.