Biomimetic coatings functionalized with adhesion peptides for dental implants
Biomimetic coatings functionalized with adhesion peptides for dental implants
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DOI：
10.1023/a:1012807621414
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发表时间：
2001-01-01
影响因子：
3.7
通讯作者：
Dard, M
中科院分区：
文献类型：
--
作者：
Roessler, S;Born, R;Dard, M
与周围组织（骨、牙龈）的完全生物整合是牙科种植体临床成功的关键步骤。在这项工作中，已经开发了由I型胶原蛋白（用于牙龈区域）和羟基磷灰石（HAP）或矿化胶原蛋白（用于骨界面）组成的仿生涂层作为关于界面的合适表面。此外，使用这些仿生涂层作为基质，结合粘附肽以进一步增加钛植入物表面的特异性。为了增强牙龈区域中的细胞附着，将从层粘连蛋白序列（TWYKIAFQRNRK）开发的线性粘附肽结合到胶原蛋白，而对于骨界面，使用适当的锚系统将环状RGD肽结合到HAP和矿化胶原蛋白。从HaCaT人角质形成细胞和MC 3 T3-E1小鼠成骨细胞的细胞附着实验中推导出的这些涂层的生物学潜力显示，牙龈区域的胶原蛋白和层粘连蛋白序列涂层以及骨接触区域的矿化胶原蛋白和RGD肽涂层的结果最好。我们的概念开辟了有前途的方法，以提高牙种植体的生物整合。(C)2001 Kluwer Academic Publishers.
A complete biological integration into the surrounding tissues (bone, gingiva) is a critical step for clinical success of a dental implant. In this work biomimetic coatings consisting either of collagen type I (for the gingiva region) and hydroxyapatite (HAP) or mineralized collagen (for the bone interface) have been developed as suitable surfaces regarding the interfaces. Additionally, using these biomimetic coatings as a matrix, adhesion peptides were bound to further increase the specificity of titanium implant surfaces. To enhance cell attachment in the gingiva region, a linear adhesion peptide developed from a laminin sequence (TWYKIAFQRNRK) was bound to collagen, whereas for the bone interface, a cyclic RGD peptide was bound to HAP and mineralized collagen using adequate anchor systems. The biological potential of these coatings deduced from cell attachment experiments with HaCaT human keratinocytes and MC3T3-E1 mouse osteoblasts showed the best results for collagen and laminin sequence coating for the gingiva region and mineralized collagen and RGD peptide coatings for regions with bone contact. Our concept opens promising approaches to improve the biological integration of dental implants. (C) 2001 Kluwer Academic Publishers.