Contractile responses in arteries subjected to hypertensive pressure in seven-day organ culture
Contractile responses in arteries subjected to hypertensive pressure in seven-day organ culture
复制标题
DOI：
10.1114/1.1376391
复制
发表时间：
2001-06-01
影响因子：
3.8
通讯作者：
Ku, DN
中科院分区：
文献类型：
--
作者：
Han, HC;Ku, DN
高血压动脉的早期变化对动脉的长期适应有显着影响。与长期适应相比，人们对高血压最初几天内高血压动脉的早期尺寸和功能变化知之甚少。为了研究高血压动脉的早期变化，在高血压（200 +/- 30 mm Hg）或正常血压（100 +/- 20 mm Hg）压力条件下，将猪颈总动脉在简化的离体动脉器官培养系统中培养7天，同时保持15 dyn/cm（2）的生理平均壁剪切应力。血管活力通过对去甲肾上腺素（NE）、卡巴胆碱（CCh）和硝普钠（SNP）的收缩直径反应以及线粒体活性和细胞凋亡/坏死的染色来证明。结果表明，动脉表现出强烈的收缩反应，NE，CCh，和SNP，基础张力，和活线粒体在器官培养系统中7天。高血压动脉收缩反应强于正常动脉（P < 0.05）。与正常血压条件下培养的动脉相比，在高血压动脉中观察到直径增大。总之，脉动培养系统可以维持动脉活力与积极的血管舒缩张力长达七天。高血压通过在最初七天内显著增加动脉直径和收缩反应来引起动脉适应。(C)2001生物医学工程学会。
Early stage changes in hypertensive arteries have a significant effect on the long-term adaptation of the arteries. Compared to the long-term adaptation, little is known about the early dimensional and functional changes in hypertensive arteries in the first few days of hypertension. To study the early stage changes in hypertensive arteries, porcine common carotid arteries were cultured for seven days in a simplified ex vivo artery organ culture system with pulsatile flow under hypertensive (200 +/- 30 mm Hg) or normotensive (100 +/- 20 mm Hg) pressure conditions while maintaining a physiological mean wall shear stress of 15 dyn/cm(2). Vessel viability was demonstrated by contractile diameter responses to norepinephrine (NE), carbachol (CCh), and sodium nitroprusside (SNP) as well as staining for mitochondrial activity and cell apoptosis/necrosis. The results show that arteries demonstrated strong contractile responses to NE, CCh, and SNP, basal tone, and viable mitochondria in the organ culture system for seven days. Hypertensive arteries demonstrated a stronger contractile response than normotensive arteries (p < 0.05). Diameter enlargement was observed in hypertensive arteries as compared to arteries cultured under normotensive conditions. In conclusion, the pulsatile culture system can maintain arteries viable with active vasomotion tone for up to seven days. Hypertensive pressure causes arterial adaptation by significantly increasing arterial diameter and contractile response within the first seven days. (C) 2001 Biomedical Engineering Society.