Screening for intestinal helminth infestation in a semi-urban cohort of HIV-infected people in Uganda: a combination of techniques may enhance diagnostic yield in the absence of multiple stool samples
Screening for intestinal helminth infestation in a semi-urban cohort of HIV-infected people in Uganda: a combination of techniques may enhance diagnostic yield in the absence of multiple stool samples
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DOI：
10.1177/004947550303300206
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发表时间：
2003-04-01
期刊：
影响因子：
0.6
通讯作者：
Whitworth, J
中科院分区：
文献类型：
--
作者：
Brown, M;Bukusuba, J;Whitworth, J
肠道蠕虫的患病率最好通过使用每个受试者的多个粪便样本来确定，但这在诊所或医院环境中可能很困难。我们在乌干达恩德培的一组412名HIV感染者中使用了一系列成熟的寄生虫学技术，研究蠕虫感染与HIV之间的相互作用。一个单一的粪便样本分析低估了蠕虫的患病率，特别是低强度感染，但加藤-卡茨涂片，福尔马林-乙醚浓度（FEC），类圆线虫和血清酶联免疫吸附试验曼氏血吸虫抗原（CAA）的炭培养相结合的诊断率增加。仅通过FEC，FEC和Kato-Katz，FEC，Kato-Katz和木炭培养的诊断率分别为23%，35%，39%和49%。对单个样本执行一系列技术可以增强寄生虫的检测。技术对不同蠕虫的敏感性不同，因此适当的技术选择取决于正在寻找的寄生虫物种。
Intestinal helminth prevalence is best determined by using multiple stool samples from each subject, but this maybe difficult in the clinic or hospital setting. We used a range of well-established parasitological techniques in a study of interactions between helminth infestation and HIV in a cohort of 412 HIV-infected people in Entebbe, Uganda. Analysis of a single stool sample underestimated helminth prevalence, especially of low-intensity infections, but a combination of Kato-Katz smear, formol-ether concentration (FEC), charcoal culture for Strongyloides and a serum enzyme-linked immunosorbent assay for Schistosoma mansoni antigen (CAA) increased diagnostic yield. Helminths were diagnosed in 23% patients by FEC alone, 35% by FEC and Kato-Katz, 39% by FEC, Kato-Katz and charcoal culture and 49% by a combination of all three tests plus CAA. Performing a range of techniques on a single sample may enhance the detection of parasites. Techniques vary in their sensitivity for different helminths so the appropriate choice of techniques depends on which parasite species are being sought.