Metabolomic and transcriptomic profiling reveals distinct nutritional properties of cassavas with different flesh colors.
Metabolomic and transcriptomic profiling reveals distinct nutritional properties of cassavas with different flesh colors.
复制标题
DOI：
10.1016/j.fochms.2021.100016
复制
发表时间：
2021-07-30
期刊：
影响因子：
--
通讯作者：
Yan H
中科院分区：
文献类型：
--
作者：
Xiao L;Cao S;Shang X;Xie X;Zeng W;Lu L;Kong Q;Yan H
在3个木薯品种中共鉴定出508种代谢产物。白肉木薯氨基酸和有机酸含量最高。黄肉木薯富含与特定途径相关的代谢物。发现几种途径在转录水平上受到调控。木薯是几个发展中国家重要的粮食安全作物。木薯根中的蛋氨酸提供了许多人类健康所必需的营养物质。利用木薯中存在的营养成分的多样性对于提高其营养价值至关重要。为解决这一问题，分别对白肉、淡黄肉和黄肉3个木薯品种的根系代谢物组进行了综合测定。在木薯根中共检测到508种代谢产物，其中初级代谢产物300种，次级代谢产物185种。3个木薯品种间有22.6%~ 34.1%的代谢产物存在显著差异。浅黄色果肉的木薯含有较高的次生代谢产物，尤其是黄酮类、苯丙素类和生物碱类，而除脂类、醇类、维生素和衍生物外，其它的初级代谢产物含量较低。与淡黄肉木薯相比，黄肉木薯的氨基酸及其衍生物含量较高，苯丙素类、核苷酸及其衍生物含量较低。白肉木薯的初级代谢产物含量较高，尤其是氨基酸及其衍生物，而次级代谢产物含量较低，但黄酮类和吲哚衍生物含量较低。随后进行转录组分析，以破译调节相关代谢物积累的潜在机制。多条代谢途径均富集了差异表达的基因和差异积累的代谢物，支持这些代谢物的代谢在转录水平上受到调控。这些结果丰富了对木薯根中代谢产物组成的认识，为高营养价值木薯的遗传改良提供了重要信息。
A total of 508 metabolites were identified in three cassava cultivars. White-fleshed cassava had the highest contents of amino acids and organic acids. Yellow-fleshed cassava was enriched in metabolites related to specific pathways. Several pathways were found to be regulated at the transcriptional level. Cassava is a significant food security crop in several developing countries. Metabolites in cassava roots provide numerous nutrients essential for human health. Exploiting the diversity of nutritional ingredients present in cassavas is vital for improving its nutritional value. To address this problem, root metabolomes of three cassava cultivars with white-flesh, light-yellow-flesh and yellow-flesh were comprehensively measured, respectively. A total of 508 metabolites were detected in cassava roots, including 300 primary metabolites and 185 secondary metabolites. There were 22.6% to 34.1% metabolites exhibiting significant variations among the three cassava cultivars. The light-yellow-flesh cassava contained higher contents of secondary metabolites, especially flavone, phenylpropanoids and alkaloids, and lower contents of primary metabolites except lipids, alcohols, vitamins and derivatives. Compared with light-yellow-flesh cassava, the yellow-flesh cassava contained higher contents of amino acid and derivatives, but lower contents of phenylpropanoids, nucleotide and derivates. White-flesh cassava contained higher contents of primary metabolites, especially amino acid and derivatives, but lower contents of secondary metabolites except flavonoid and indole derivatives. Transcriptome analyses were parallelly performed to decipher the potential mechanisms regulating the accumulations of related metabolites. Several pathways were both enriched by differentially expressed genes and differentially accumulated metabolites, supporting that metabolisms of these metabolites were regulated at transcriptional level. These results expand the knowledge on metabolite compositions in cassava roots and provide substantial information for genetic improvement of cassavas with high nutritional values.