Analysis of tumor suppressor gene on human chromosome 9 in mouse x human somatic cell hybrids.
Analysis of tumor suppressor gene on human chromosome 9 in mouse x human somatic cell hybrids.
复制标题
小鼠 x 人体细胞杂交体中人 9 号染色体上的肿瘤抑制基因分析。
DOI：
10.1007/bf02257456
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Diaz,MO
中科院分区：
文献类型：
--
作者：
Porterfield,BW;Olopade,OI;Rowley,JD;Diaz,MO
人类9号染色体短臂（9p）的缺失在人类白血病和实体瘤中很常见。这些缺失的最小重叠区域位于干扰素基因和甲硫腺苷磷酸化酶基因之间，与小鼠4号染色体上具有肿瘤抑制活性的区域部分同线。在需要MTAP活性以继续生长的培养条件下，选择致瘤性、MTAP缺陷型小鼠L细胞和含有9p正常拷贝或含有涉及条带9p21的缺失的9p的MTAP感受态人细胞之间的体细胞杂交体。含有9p正常拷贝的体细胞杂交体很少在裸鼠中形成肿瘤。生长的罕见肿瘤细胞失去了正常的9p。含有9p缺失的杂交细胞更频繁地形成肿瘤，并且来自这些肿瘤的细胞保留了9p缺失染色体。这些结果提供了一个（或多个）肿瘤抑制基因位于人类9号染色体缺失区域内的证据。
Deletions of the short arm of human chromosome 9 (9p) are common in human leukemia and solid tumors. The minimum region of overlap of these deletions, located between the interferon genes and the methylthioadenosine phosphorylase gene, is partially syntenic with a region of mouse chromosome 4 that has tumor suppressor activity. Somatic cell hybrids between tumorigenic, MTAP-deficient, mouse L cells, and MTAP-competent human cells containing either a normal copy of 9p or a 9p with a deletion involving band 9p21 were selected in culture conditions that require MTAP activity for continued growth. Somatic cell hybrids that contained a normal copy of 9p rarely formed tumors in nude mice. Cells from the rare tumors that grew had lost the normal 9p. Hybrid cells that contained a 9p with deletions formed tumors more frequently, and cells from these tumors retained the 9p deletion chromosome. These results provide evidence that a tumor suppressor gene (or genes) is located on human chromosome 9 within the region of deletion.