In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.
In-vitro recombination in rad and rnc mutants of Saccharomyces cerevisiae.
复制标题
酿酒酵母 rad 和 rnc 突变体的体外重组。
DOI：
10.1007/bf00336741
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发表时间：
1993
期刊：
影响因子：
2.5
通讯作者：
Chow,TY
中科院分区：
文献类型：
--
作者：
Moore,PD;Simon,JR;Wallace,LJ;Chow,TY
S.体外实验表明，细胞能催化质粒间的同源重组。与野生型相比，从rad50、rad52或rad54破坏突变体制备的提取物都具有降低的重组活性。有52和54种提取物在含有双链断裂的质粒的重组中比完整质粒更受损，而50种提取物对两种类型的底物同样缺乏。核酸酶RhoNuc（以前命名为yNucR）由RNC1（以前命名为NUC2）基因编码并由RAD 52基因调节，当一种底物是单链时，它不是重组所必需的，但当两种底物都是双链时，它是大多数重组事件所必需的。此外，这种核酸酶的消除恢复重组inrad52提取物的水平与野生型提取物中的那些相当。
Extracts ofS. cerevisiaecells can catalyze homologous recombination between plasmids in vitro. Extracts prepared fromrad50, rad52orrad54disruption mutants all have reduced recombinational activity compared to wild-type. Therad52andrad54extracts are more impaired in the recombination of plasmids containing double-strand breaks than of intact plasmids, whereasrad50extracts are deficient equally for both types of substrate. The nuclease RhoNuc (previously designated yNucR), encoded by theRNC1(previously designatedNUC2) gene and regulated by theRAD52gene, is not required for recombination when one substrate is single-stranded but is essential for the majority of recombination events when both substrates are double-stranded. Furthermore, elimination of this nuclease restores recombination inrad52extracts to levels comparable to those in wild-type extracts.