Expression of mRNAs for neurotrophins and their receptors in the rat choroid plexus and dura mater.
Expression of mRNAs for neurotrophins and their receptors in the rat choroid plexus and dura mater.
复制标题
大鼠脉络丛和硬脑膜中神经营养因子及其受体的 mRNA 表达。
作者：
T. Timmusk;G. Mudó;M. Metsis;N. Belluardo
用核糖核酸酶保护试验研究了成年大鼠脉络丛和硬脑膜中神经营养因子、神经生长因子、脑源性神经营养因子、神经营养因子-3和神经营养因子-4（NGF、BDNF、NT-3和NT-4）及其酪氨酸激酶家族受体（trkA、trkB和trkC）的存在。脉络丛含有高水平的NGF和NT-4 mRNA，低水平的NT-3和BDNF mRNA;高水平的trkB mRNA，以及检测不到的trkA和trkC mRNA水平。在硬脑膜中有高水平的NT-3和NGF，低水平的BDNF和NT-4 mRNA，以及高水平的trkC mRNA和相对高量的trkB mRNA，而trkA mRNA的水平是不可检测的。本分析揭示了不同的分布的神经营养素及其相关受体在脉络丛和硬脑膜。
The presence of the neurotrophin, nerve growth factor, brain derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 (NGF, BDNF, NT-3 and NT-4) and their receptors of the tyrosine kinase family (trkA, trkB and trkC) have been investigated in the choroid plexus and dura mater of the adult rat by ribonuclease protection assay. The choroid plexus contained high levels of mRNAs for NGF and NT-4, and low levels of NT-3 and BDNF mRNA; and high levels of trkB mRNA, and undetectable levels of trkA and trkC mRNA. In the dura mater there were high levels of NT-3 and NGF, and low levels of BDNF and NT-4 mRNAs; and high levels of trkC mRNA, and relatively high amount of trkB mRNA, while levels of trkA mRNA was undetectable. The present analysis revealed a different distribution of neurotrophins and their related receptors in the choroid plexus and dura mater.