CELL-CELL INTERACTIONS IN CONJUGATING ESCHERICHIA-COLI - ROLE OF F-PILI AND FATE OF MATING AGGREGATES
CELL-CELL INTERACTIONS IN CONJUGATING ESCHERICHIA-COLI - ROLE OF F-PILI AND FATE OF MATING AGGREGATES
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DOI：
10.1128/jb.135.3.1053-1061.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
SCHWUCHOW, S
中科院分区：
文献类型：
--
作者：
ACHTMAN, M;MORELLI, G;SCHWUCHOW, S
Bacterial conjugation between E. coli cells was investigated by a combination of physical and genetic techniques, using Hfr, F'', or R+ donors and F- recipients. DNA transfer occurred in mating aggregates of up to 50 cells. Multiple interactions between donor and recipient cells occurred, and both F-pilus connections and wall-to-wall contacts were detectable. The detectable F-pilus contacts could be destroyed without either disrupting the mating aggregates or preventing DNA transfer. Hfr .times. F- mating aggregates did not disaggregate even though recombinant frequencies were inversely proportional to the distance from the origin of DNA transfer. F'' or R+ donors formed mating aggregates with F- cells which disaggregated soon after transfer of the autonomous sex factor DNA.
