The oral administration of silver nanoparticles activates the kynurenine pathway in rat brain independently of oxidative stress
The oral administration of silver nanoparticles activates the kynurenine pathway in rat brain independently of oxidative stress
复制标题
DOI：
10.1016/j.cbi.2019.01.034
复制
发表时间：
2019-04-01
影响因子：
5.1
通讯作者：
Otohinoyi, David Adeiza
中科院分区：
文献类型：
--
作者：
Adeyemi, Oluyomi Stephen;Uloko, Rhoda Ananu;Otohinoyi, David Adeiza
在这项工作中，我们确定氧化应激是否有助于AgNPs激活犬尿氨酸途径。体重130 ~ 146 g的雄性Wistar大鼠随机分为6组。阴性对照组灌胃蒸馏水，其余处理组分别单用AgNPs（25、50 mg/kg bw）或联用Trolox （100 mg/kg bw）。结果表明，AgNPs处理显著提高了大鼠肝脏中蛋白羰基水平，而与Trolox共同处理则降低了升高。相反，与阴性对照相比，AgNPs提高了大鼠血浆和组织中的还原性谷胱甘肽（GSH）水平。此外，与阴性对照组相比，口服AgNPs （50 mg/kg bw）显著提高了大鼠血浆和脑犬尿氨酸水平。同时，与Trolox联合治疗可明显恢复大鼠血浆中的犬尿氨酸水平，但不能恢复大鼠脑中的犬尿氨酸水平。综上所述，研究结果表明，在本研究中使用的剂量下，口服AgNPs可能不会引起氧化应激。然而，与Trolox共同治疗似乎增强了暴露于AgNPs后大鼠的氧化应激。此外，数据支持AgNPs激活大鼠脑中犬尿氨酸通路可能与氧化应激无关。这些发现是新的，有助于加深我们对纳米颗粒细胞相互作用的理解。
In this work, we determined whether oxidative stress contributed to the activation of the kynurenine pathway by AgNPs. Male Wistar rats weighing between 130 and 146 g were randomly assigned into six groups. Animals in the negative control group were orally administered distilled water while, the other treatment groups were respectively given AgNPs (25 and 50 mg/kg bw) alone or in combination with Trolox (100 mg/kg bw). Results showed that treatments with AgNPs significantly raised protein carbonyl level in rat liver, but the co-treatment with Trolox attenuated the elevation. Conversely, AgNPs raised the level of reduced glutathione (GSH) in rat plasma and tissues compared to the negative control. Further, oral exposure to AgNPs (50 mg/kg bw) significantly elevated rat plasma and brain kynurenine levels compared to the negative control. Meantime, the co-treatment with Trolox appreciably restored kynurenine level in rat plasma, but not in the rat brain. Taken together, findings indicate that the oral administration of AgNPs alone at the doses used in this study, might not have caused oxidative stress. However, the co-treatment with Trolox appears to potentiate oxidative stress in rats following exposure to AgNPs. Furthermore, data support that the activation of the kynurenine pathway in the rat brain by AgNPs might be independent of oxidative stress. The findings are new and contribute to deepen our understanding of the cellular interaction by nanoparticles.