Phospholipid monolayer coated microfabricated electrodes to model the interaction of molecules with biomembranes
Phospholipid monolayer coated microfabricated electrodes to model the interaction of molecules with biomembranes
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DOI：
10.1016/j.electacta.2009.02.095
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发表时间：
2009-09-01
影响因子：
6.6
通讯作者：
Nelson, Andrew
中科院分区：
文献类型：
--
作者：
Coldrick, Zachary;Steenson, Paul;Nelson, Andrew
悬汞滴电极（HMDE）由于其原子级光滑的表面和零电荷电位下的疏水性而作为研究表面有机膜的吸附和脱附过程的工具具有经典应用。在这项研究中，我们报告的替代HMDE研究支持的有机层的形式，铂（Pt）的工作电极制造使用光刻技术，其上的薄膜的汞是电沉积。这些基于晶片的Pt/Hg电极的特征在于，并使用快速循环伏安法（RCV）与HMDE进行比较，显示出相似的电容-电位曲线，同时具有更高的机械稳定性，并且在几个月的寿命内消耗的汞显著减少。电极已被用来支持自组装磷脂单层，这是动态的表面涂层具有独特的介电性能。通过在磷脂涂层之前施加比-2.6V（相对于Ag/AgCl）更负的极端阴极电位来再生电极表面，解决了表面污染问题。本文提出的磷脂涂层电极模拟磷脂双层的一半，并表现出与生物膜活性药物分子氯丙嗪和奎尼丁的相互作用。这些相互作用的幅度已被评估，通过记录在真实的时间的电容-电位曲线的变化，使用RCV在40 V s（-1）超过1 V的电位范围。一种方法，电极涂层与磷脂的电极安装在流动池设备已经开发。这使得能够进行连续的快速清洁/涂覆/相互作用循环，用于药物筛选和/或在线监测感兴趣的分子。(C)2009爱思唯尔有限公司保留所有权利。
The hanging mercury (Hg) drop electrode (HMDE) has a classical application as a tool to study adsorption and desorption processes of surface organic films due to its: (a) atomically smooth surface and, (b) hydrophobicity at its potential of zero charge. In this study we report on a replacement of the HMDE for studying supported organic layers in the form of platinum (Pt) working electrodes fabricated using lithography techniques on which a thin film of Hg is electrodeposited. These wafer-based Pt/Hg electrodes are characterised and compared to the HMDE using rapid cyclic voltammetry (RCV) and show similar capacitance-potential profiles while being far more mechanically stable and consuming considerably less Hg over their lifetime of several months. The electrodes have been used to support self-assembled phospholipid monolayers which are dynamic surface coatings with unique dielectric properties. The issue of surface contamination has been solved by regenerating the electrode surface prior to phospholipid coating by application of extreme cathodic potentials more negative than -2.6V (vs. Ag/AgCl). The phospholipid coated electrodes presented in this paper mimic one half of a phospholipid bilayer and exhibit interactions with the biomembrane active drug molecules chlorpromazine, and quinidine. The magnitudes of these interactions have been assessed by recording changes in the capacitance-potential profiles in real time using RCV at 40 V s(-1) over potential ranges >1 V. A method for electrode coating with phospholipids with the electrodes fitted in a flow cell device has been developed. This has enabled sequential rapid cleaning/coating/interaction cycles for the purposes of drug screening and/or on-line monitoring for molecules of interest. (C) 2009 Elsevier Ltd. All rights reserved.