Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.
Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.
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DOI：
10.1016/j.tranon.2016.02.001
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发表时间：
2016-04
影响因子：
5
通讯作者：
Laterra J
中科院分区：
文献类型：
--
作者：
Goodwin CR;Woodard CL;Zhou X;Pan J;Olivi A;Xia S;Bettegowda C;Sciubba DM;Pevsner J;Zhu H;Laterra J
蛋白质微阵列技术已成功用于鉴定纯化的活化激酶的底物。我们使用蛋白质微阵列全面探讨 PTEN 和 Akt 活性对体外和体内神经胶质瘤模型磷酸激酶组的影响，并在临床病理标本中验证结果。从肿瘤样品中提取的全细胞裂解物可应用于人类激酶组芯片微阵列，以高通量方式分析全局激酶磷酸化模式，并鉴定肿瘤细胞固有的新底物以及与肿瘤微环境的相互作用。我们的研究结果确定了一种基于微阵列的新型方法，用于评估适用于人类肿瘤发生和其他病理生理状态的细胞内信号传导事件。
Protein microarray technology has been successfully used for identifying substrates of purified activated kinases. We used protein microarrays to globally interrogate the effects of PTEN and Akt activity on the phospho-kinome of in vitro and in vivo glioma models and validated results in clinical pathological specimens. Whole cell lysates extracted from tumor samples can be applied to human kinome chip microarrays to profile the global kinase phosphorylation patterns in a high-throughput manner and identify novel substrates inherent to the tumor cell and the interactions with tumor microenvironment. Our findings identify a novel microarray-based method for assessing intracellular signaling events applicable to human oncogenesis and other pathophysiologic states.