Histochemical identification of three types of intrafusal muscle fibers in the cat and monkey based on the myosin ATPase reaction.
Histochemical identification of three types of intrafusal muscle fibers in the cat and monkey based on the myosin ATPase reaction.
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基于肌球蛋白 ATP 酶反应对猫和猴三种类型的梭内肌纤维进行组织化学鉴定。
DOI：
10.1139/y72-030
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发表时间：
1972
影响因子：
2.1
通讯作者：
R. S. Smith
中科院分区：
文献类型：
--
作者：
W. Ovalle;R. S. Smith
用最近描述的肌球蛋白atp酶反应的修饰对猫和猴子的蚓状肌的连续冷冻切片进行了测试（Guth和Samaha 1970）。Exp. Neurol 28,365)。该反应能够检测酸稳定、碱不稳定的肌球蛋白atp酶和酸不稳定、碱稳定的肌球蛋白atp酶。在pH为10.4或4.35的条件下，在pH为9.4的底物中进行预孵育反应。根据对肌球蛋白atp酶的反应，可以在猫和猴体内检测到三种类型的肌内肌纤维。核袋纤维可分为两种类型：一种只含有肌球蛋白atp酶的酸稳定形式，另一种含有在酸和碱条件下均可检测到的atp酶。核链纤维只含有碱稳定形式的atp酶。
Serial frozen section of muscle spindles in the lumbrical muscles of the cat and the monkey were tested with a recently described modification of the myosin ATPase reaction (Guth and Samaha 1970. Exp. Neurol 28, 365). This reaction is able to detect acid-stable, alkaline-labile myosin ATPase and acid-labile, alkaline-stable myosin ATPase. The reactions were carried out by preincubation of the sections at pH 10.4 or 4.35 with incubation in a substrate at pH 9.4. In both the cat and the monkey three types of intrafusal muscle fibers could be detected on the basis of their reaction for myosin ATPase. Nuclear bag fibers could be divided into two types: those containing the acid-stable form of myosin ATPase only, and those containing ATPase detectable under both acid and alkaline conditions. Nuclear chain fibers contained the alkaline-stable form of ATPase only.