ISOLATION OF A MULTIENZYME COMPLEX OF FATTY-ACID OXIDATION FROM ESCHERICHIA-COLI
ISOLATION OF A MULTIENZYME COMPLEX OF FATTY-ACID OXIDATION FROM ESCHERICHIA-COLI
复制标题
DOI：
10.1073/pnas.74.2.492
复制
发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
SCHULZ, H
中科院分区：
文献类型：
--
作者：
BINSTOCK, JF;PRAMANIK, A;SCHULZ, H
从大肠杆菌B细胞中分离出脂肪酸氧化的多酶复合体，并用简单的两步法将其纯化到接近均一的水平。该络合物对短链、中链和长链底物具有硫解酶(EC 2.3.1.9)、Enoyl-CoA水合酶(EC 4.2.1.17)和3-羟基酰基-CoA脱氢酶(EC 1.1.35)活性。复杂的mw是.apprx。300,000，明显由两种亚基组成，分子量分别为78,000和42,000。
A multi-enzyme complex of fatty acid oxidation was isolated from E. coli B cells and was purified to near homogeneity by a simple 2-step procedure. The complex exhibits thiolase (EC 2.3.1.9), enoyl-CoA hydratase (EC 4.2.1.17) and 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) activities towards short-, medium- and long-chain substrates. The complex MW is .apprx. 300,000 and is apparently composed of 2 types of subunits with MW of 78,000 and 42,000, respectively.