Chemical aspects of clustered DNA damage induction by ionising radiation
Chemical aspects of clustered DNA damage induction by ionising radiation
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DOI：
10.1093/oxfordjournals.rpd.a006840
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发表时间：
2002-01-01
影响因子：
1
通讯作者：
O'Neill, P
中科院分区：
文献类型：
--
作者：
Lomax, ME;Gulston, MK;O'Neill, P
电离辐射诱导DNA的各种化学修饰，从简单的，孤立的损伤到成簇的DNA损伤。其中两个或多个损伤通过单个辐射轨迹在几十个碱基对内形成。多发性病变，例如串联病变和损伤扩大。也可以通过与单个羟基自由基的反应A在DNA中诱导。根据生物物理模型，已经提出成簇的DNA损伤较难修复，因此有助于电离辐射的生物严重性。在这篇综述中，一些证据表明，非双链断裂（non-DSB）集群DNA损伤诱导显着的产量，相对于DSB，在哺乳动物细胞。在合成寡核苷酸中成簇DNA损伤的酶促处理已被证明是受损的，这取决于存在的病变的性质。成簇DNA损伤在辐射诱发的癌变发展早期阶段的作用仍有待探讨。
Ionising radiation induces a variety of chemical modifications to DNA, ranging from simple, isolated lesions to clustered DNA damage. in which two or more lesions are formed within a few tens of base pairs by a single radiation track. Multiple lesions, e.g. tandem lesions and amplification of damage. may also be induced in DNA by reaction A with a single hydroxyl radical, It has been proposed from biophysical modelling that clustered DNA damage is less repairable and therefore contributes to the biological severity of ionising radiation. In this review, some evidence is presented which indicates that non-double strand break (non-DSB) clustered DNA damage is induced in significant yield, relative to that of DSBs, in mammalian cells. Enzymatic processing of clustered DNA damage in synthetic oligonucleotides has been shown to be compromised, depending on the nature of the lesions present. The role of clustered DNA damage in the early stages of the development of radiation-induced carcinogenesis remains to be addressed.