Electron tomography reveals a flared morphology on growing microtubule ends
Electron tomography reveals a flared morphology on growing microtubule ends
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DOI：
10.1242/jcs.072967
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发表时间：
2011-03-01
影响因子：
4
通讯作者：
Brunner, Damian
中科院分区：
文献类型：
--
作者：
Hoeoeg, Johanna L.;Huisman, Stephen M.;Brunner, Damian
微管（MTs）表现出动态不稳定性，在生长和缩短阶段之间交替，主要是在它们的无帽正端。低温电镜结果表明，生长中的MTs主要表现为弯曲片和钝端；在解聚过程中，卷曲的“拉姆香”占主导地位。MTs的观察有丝分裂细胞表明,体内的情况不同于体外,但到目前为止,一个清晰的体内和体外结果之间的比较是不可能的因为太结束结构不能直接与特定的动态相关。在这里我们结合光学显微镜和电子断层扫描(ET)增长显示MT +结束裂殖酵母粟酒裂殖酵母显示主要喇叭形态。这表明MT在体内和体外的聚合可以遵循不同的途径。
Microtubules (MTs) exhibit dynamic instability, alternating between phases of growth and shortening, mostly at their uncapped plus ends. Based on results from cryo-electron microscopy it was proposed that growing MTs display mainly curved sheets and blunt ends; during depolymerisation curled 'ramshorns' predominate. Observations of MTs in mitotic cells have suggested that the situation in vivo differs from that in vitro, but so far, a clear comparison between in vivo and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display predominantly a flared morphology. This indicates that MT polymerisation in vivo and in vitro can follow different paths.