Parametric study of a microdialysis probe and study of depletion effect using ethanol as a test analyte.
Parametric study of a microdialysis probe and study of depletion effect using ethanol as a test analyte.
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微透析探针的参数研究以及使用乙醇作为测试分析物的消耗效应研究。
DOI：
10.1016/j.bbrc.2022.10.086
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发表时间：
2022
影响因子：
3.1
通讯作者：
Hutter,Tanya
中科院分区：
文献类型：
--
作者：
Lee,Tse-Ang;Gonzales,Rueben;Hutter,Tanya
虽然微透析是一种常见的体内取样技术，但用于乙醇分子取样的微透析探针的性能的详细表征尚未进行。在这项工作中，进行了实验和计算研究，定量地研究了自制和市售探针的乙醇扩散特性。探针效率，即回收率（定义为透析液中乙醇浓度与探针周围外部介质中乙醇浓度的比值）用于表征性能。在可控的环境条件下，测定不同灌注流速（0.1、0.2、0.5、1、1.5、2 μL/min）和外源乙醇浓度（1、2.5、5、10、20 mM）下的回收率。在19℃、37℃和47℃时考察了温度的影响。结果表明，当流速从2 μL/min降低到0.1 μL/min时，探针的回收率至少提高了3倍，且随外源乙醇浓度的变化回收率基本保持不变。两种不同膜材料和结构的商业微透析探针的性能也进行了测试，并具有相似的回收率。通过计算建模，拟合实验数据，确定乙醇在自制探针半透膜中的扩散系数为9 × 1011m2/s （R2> 0.99）。此外，数值模拟了不同流速下的耗尽效应随时间的变化以及体内乙醇清除率，结果表明，当流速低于1 μL/min时，耗尽区明显缩小。该结果有助于更好地理解微透析探针在乙醇取样时的扩散特性，可用于更好地解释微透析探针的内渗测量和优化微透析探针。
Although microdialysis is a commonin vivosampling technique, a detailed characterization of the performance of a microdialysis probe used for sampling ethanol molecules has not been conducted. In this work, experimental and computational investigations were carried out to quantitatively study ethanol diffusion characteristics for home-made and commercially available probes. Probe efficiency, i.e. recovery rate (defined as the ethanol concentration in the dialysate to that in the external medium surrounding the probe) was used to characterize the performance. The recovery rate was measured at different perfusion flow rates (0.1, 0.2, 0.5, 1, 1.5, 2 μL/min) and external ethanol concentrations (1, 2.5, 5, 10, 20 mM) with controlled environmental conditions. Effect of temperature was also investigated at 19, 37 and 47 °C. The results show that reducing the flow rate from 2 to 0.1 μL/min at least triples the recovery rate for the home-made probes, and it remains nearly unchanged when varying external ethanol concentration. The performance for two commercial microdialysis probes with different membrane materials and configurations were also determined and have similar recovery rates. Through computational modeling, the diffusion coefficient of ethanol in the semipermeable membrane of the home-made probe was determined by fitting the experimental data, and it was found to be 9 × 1011m2/s (R2> 0.99). In addition, the depletion effect over time at different flow rates along with estimatedin vivoethanol clearance were simulated numerically, showing that the depletion region shrinks significantly when the flow rate is below 1 μL/min. The results provide better understanding of the diffusion characteristics of the microdialysis probe when used for sampling ethanol which can be used for better interpretation ofin vivomeasurements and for microdialysis probe optimization.