WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis
WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis
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DOI：
10.1038/s41467-019-13074-9
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发表时间：
2019-11-08
影响因子：
16.6
通讯作者：
Lohmann, Jan U.
中科院分区：
文献类型：
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作者：
Ma, Yanfei;Miotk, Andrej;Lohmann, Jan U.
为了维持干细胞自我更新和分化之间的长期平衡，动态信号需要被翻译成空间精确和时间稳定的基因表达状态。在植物顶端干细胞系统中，局部积累的小而高流动性的植物激素生长素触发分化，同时，多能性干细胞在整个生命周期中保持不变。我们发现干细胞对生长素介导的分化有抗性，但需要低水平的信号传导来维持。我们证明，WUSCHEL转录因子通过流变控制生长素信号和反应途径赋予这种行为。最后，我们发现WUSCHEL通过调控靶位点的组蛋白乙酰化起作用，包括那些在生长素途径中起作用的位点。我们的研究结果揭示了一种重要的机制，该机制允许细胞将有效的、高度动态的发育信号差异地转化为具有高空间精度和时间鲁棒性的稳定细胞行为。
To maintain the balance between long-term stem cell self-renewal and differentiation, dynamic signals need to be translated into spatially precise and temporally stable gene expression states. In the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers differentiation while at the same time, pluripotent stem cells are maintained throughout the entire life-cycle. We find that stem cells are resistant to auxin mediated differentiation, but require low levels of signaling for their maintenance. We demonstrate that the WUSCHEL transcription factor confers this behavior by rheostatically controlling the auxin signaling and response pathway. Finally, we show that WUSCHEL acts via regulation of histone acetylation at target loci, including those with functions in the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly dynamic developmental signal into stable cell behavior with high spatial precision and temporal robustness.