Effects of endocrine disrupting substance on estrogen receptor gene transcription in dialysis patients
Effects of endocrine disrupting substance on estrogen receptor gene transcription in dialysis patients
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DOI：
10.1111/j.1744-9987.2007.00472.x
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发表时间：
2007-08-01
影响因子：
1.9
通讯作者：
Suzuki, Hiromichi
中科院分区：
文献类型：
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作者：
Kanno, Yoshihiko;Okada, Hirokazu;Suzuki, Hiromichi
双酚A(BPA)和邻苯二甲酸酯，两种众所周知的内分泌干扰物质(EDSS)，被证明是从血液透析(HD)和腹膜透析(PD)患者使用的透析管中洗脱出来的。由于这些患者需要透析才能生存，他们可能会暴露在潜在有害水平的这些化合物中。本研究采用酶联免疫吸附试验(EL ISA)试剂盒对45例HD患者、43例PD患者和12例健康对照的血清BPA水平进行了测定。结果表明，HD(5.3+/-0.3 ng/mL)和PD(3.8+/-0.2 ng/mL)患者血清BPA水平均显著高于对照组(2.6±0.1 ng/mL；P<0.05)，且HD患者的BPA水平显著高于PD患者(P<0.05)。为了研究这些较高的血清BPA水平的潜在影响，使用荧光素酶检测系统检测了患者的血清样本对雌激素受体基因转录水平的影响。将转雌激素反应元件(ERE)基因的MCF-7细胞与患者血清或双酚A溶液共同培养。我们的结果表明，我们的患者血清诱导的ERE转录水平高于相同剂量的BPA；这种高表达可能是由于透析患者中存在其他EDSS，如邻苯二甲酸酯(DEHP)，尽管这一点仍有待确定。
Bisphenol A (BPA) and phthalate diesters, two well-described endocrine-disrupting substances (EDSs), were shown to elute out of the dialysis tubing used by patients who underwent hemodialysis (HD) and peritoneal dialysis (PD). Since these patients require dialysis for survival, they may be exposed to potentially harmful levels of these compounds. In this study, serum BPA levels were quantified in HD (n = 45) and PD (n = 43) patients, and healthy controls (n = 12) using an enzyme-linked immunosorbent assay (ELISA) kit. Our results showed that serum BPA levels were significantly elevated in both HD (5.3 +/- 0.3 ng/mL) and PD (3.8 +/- 0.2 ng/mL) patients compared to controls (2.6 0.1 ng/mL; P < 0.05); levels in the HD patients were significantly greater than in the PD patients (P < 0.05). To investigate the potential effects of these higher serum BPA levels, the patients' serum samples were examined for their effects on estrogen receptor gene transcription levels using a luciferase assay system. MCF-7 cells that were transfected with estrogen response element (ERE) cDNA were cultured with our patients' sera or a solution of BPA. Our results showed that our patients' sera induced higher levels of ERE transcription than did the same dose of BPA; this higher expression may have been due to the presence of other EDSs in the dialysis patients, such as phthalate diesters (DEHP), though this remains to be determined.