Effects of probiotic Lactobacillus paracasei TD3 on moderation of cholesterol biosynthesis pathway in rats
Effects of probiotic Lactobacillus paracasei TD3 on moderation of cholesterol biosynthesis pathway in rats
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DOI：
10.22038/ijbms.2019.33933.8073
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发表时间：
2019-09-01
影响因子：
2.2
通讯作者：
Fahimi, Hossein
中科院分区：
文献类型：
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作者：
Dehkohneh, Abolfazl;Jafari, Parvaneh;Fahimi, Hossein
目的：快餐等高脂肪食物的普遍消费是高胆固醇血症的主要原因之一，高胆固醇血症可导致心血管疾病。3-羟基-3-甲基戊二酰辅酶A还原酶（HMGCR）和细胞色素P450 7A 1（CYP 7A 1）是胆固醇代谢的两个关键基因。在饮食中使用益生菌是一种很有前途的调节血脂的方法。为了证实益生菌对血脂的调节作用，本研究检测了副干酪乳杆菌TD 3对血脂的改善作用。材料与方法：21只雄性Wistar大鼠随机分为3组（n=7）。第一组：正常饮食的阴性对照，G2：高脂饮食的阳性对照，G3 T：高脂饮食加补充副干酪乳杆菌TD 3（10（10）CFU）的试验组。第21天，用氯仿麻醉大鼠，然后处死。采集血液样本，通过自动分析仪系统分析血脂参数和肝酶。采用实时荧光定量PCR方法检测脂肪组织中HMGCR和CYP 7A 1基因的表达。副干酪素TD 3能显著降低血清胆固醇水平（P
Objective(s): Prevalence of high-fat food consumption, such as fast foods is one of the major causes of hypercholesterolemia, which can lead to cardiovascular diseases. 3-hydroxy-3-methylglutarylcoenzyme A reductase (HMGCR) and cytochrome P450 7A1 (CYP7A1) are two key genes in cholesterol metabolism. Use of probiotics in the diet is a promising approach for modulation of serum lipid. To confirm the modulation of serum lipids by probiotics, in this study, we have examined the efficacy of Lactobacillus paracasei TD3 in improving blood cholesterol levels.Materials and Methods: 21 male Wistar rats were divided into three groups randomly (n=7). G1: negative control with normal diet, G2: positive control with high-fat diet, G3T: test group with high-fat diet plus supplementation with L paracasei TD3 (10(10) CFU). In the 21st day, the rats were anesthetized using chloroform and then sacrificed. Blood samples were collected to analyze lipid panel parameters and hepatic enzymes by the auto-analyzer system. Adipose tissue samples were analyzed using real-time PCR for HMGCR and CYP7A1 genes expression.Results: Consumption of L. paracasei TD3 could reduce serum cholesterol levels significantly (P