A novel risk stratification model based on the Children's Hepatic Tumours International Collaboration-Hepatoblastoma Stratification and deoxyribonucleic acid methylation analysis for hepatoblastoma.
A novel risk stratification model based on the Children's Hepatic Tumours International Collaboration-Hepatoblastoma Stratification and deoxyribonucleic acid methylation analysis for hepatoblastoma.
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基于儿童肝脏肿瘤国际合作-肝母细胞瘤分层和肝母细胞瘤脱氧核糖核酸甲基化分析的新型风险分层模型。
DOI：
10.1016/j.ejca.2022.06.013
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Taketomi A.
中科院分区：
文献类型：
--
作者：
Kondo T;Honda S;Suzuki H;Ito YM;Kawakita I;Okumura K;Ara M;Minato M;Kitagawa N;Tanaka Y;Tanaka M;Shinkai M;Hishiki T;Watanabe K;Ida K;Takatori A;Hiyama E;Taketomi A.
肝母细胞瘤（HB）是儿科最常见的肝脏肿瘤，表观遗传畸变可能在HB的发展中起重要作用。最近，儿童肝肿瘤国际合作-肝母细胞瘤分层（CHIC-HS）开发了基于临床病理因素的风险分层。本研究旨在构建一个更准确的模型，通过整合CHIC-HS与分子因素的基础上DNA methylation.MethodsHB肿瘤标本（N = 132），从日本小儿肝肿瘤组-2协议治疗的患者进行收集和亚硫酸氢盐焦磷酸测序进行甲基化分析。回顾性分析了甲基化状态与临床病理因素、总生存期（OS）和无事件生存期（EFS）之间的关系。我们调查了甲基化状态的评估在每个CHIC-HS风险组的有效性，并产生了一个新的风险分层model.ResultsMost标本（82%）是从化疗后的组织。4个基因（RASSF 1A、PARP 6、OCIAD 2和MST 1 R）中≥2个基因的超甲基化与OS和EFS较差显著相关。多因素分析表明，≥2个甲基化基因是独立的预后因素（OS和EFS的风险比分别为6.014和3.684）。在CHIC-极低（VL）-/低（L）-风险和CHIC-中等（I）风险组中，两个或多个甲基化基因也与OS较差相关（3年OS率分别为83% vs. 98%和50% vs. 95%）。VL/L，我，和高风险组在新的分层模型的3年OS率分别为98%，90%和62%（与CHIC-HS [96%，82%，和65%，分别]），优化CHIC-HS.ConclusionsOur建议的分层系统认为HB的个人风险，并可能提高患者的临床管理。
IntroductionHepatoblastoma (HB) is the most common paediatric liver tumour, and epigenetic aberrations may be important in HB development. Recently, the Children's Hepatic Tumors International Collaboration-Hepatoblastoma Stratification (CHIC-HS) developed risk stratification based on clinicopathological factors. This study aimed to construct a more accurate model by integrating CHIC-HS with molecular factors based on DNA methylation.MethodsHB tumour specimens (N = 132) from patients treated with the Japanese Pediatric Liver Tumors Group-2 protocol were collected and subjected to methylation analysis by bisulfite pyrosequencing. Associations between methylation status and clinicopathological factors, overall survival (OS), and event-free survival (EFS) were retrospectively analysed. We investigated the effectiveness of the evaluation of methylation status in each CHIC-HS risk group and generated a new risk stratification model.ResultsMost specimens (82%) were from post-chemotherapy tissue. Hypermethylation in ≥2 of the four genes (RASSF1A,PARP6,OCIAD2, andMST1R) was significantly associated with poorer OS and EFS. Multivariate analysis indicated that ≥2 methylated genes was an independent prognostic factor (hazard ratios of 6.014 and 3.684 for OS and EFS, respectively). Two or more methylated genes was also associated with poorer OS in the CHIC-very low (VL)-/low (L)-risk and CHIC-intermediate (I) risk groups (3-year OS rates were 83% vs. 98% and 50% vs. 95%, respectively). The 3-year OS rates of the VL/L, I, and high-risk groups in the new stratification model were 98%, 90%, and 62% (vs. CHIC-HS [96%, 82%, and 65%, respectively]), optimising CHIC-HS.ConclusionsOur proposed stratification system considers individual risk in HB and may improve patient clinical management.
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DOI：
10.1016/s1470-2045(16)30598-8
发表时间：
2017-01
期刊：
The Lancet. Oncology
影响因子：
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作者：
Meyers RL;Maibach R;Hiyama E;Häberle B;Krailo M;Rangaswami A;Aronson DC;Malogolowkin MH;Perilongo G;von Schweinitz D;Ansari M;Lopez-Terrada D;Tanaka Y;Alaggio R;Leuschner I;Hishiki T;Schmid I;Watanabe K;Yoshimura K;Feng Y;Rinaldi E;Saraceno D;Derosa M;Czauderna P
通讯作者：
Czauderna P
德国儿童肝脏肿瘤合作研究中肝母细胞瘤的治疗。
DOI：
10.2741/395
发表时间：
2012
期刊：
Frontiers in bioscience
影响因子：
--
作者：
B. Haeberle;D. Schweinitz
通讯作者：
D. Schweinitz
影响因子：
8.4
作者：
Cairo, Stefano;Armengol, Carolina;Kappler, Roland
通讯作者：
Kappler, Roland
影响因子：
1.8
作者：
Hishiki, Tomoro;Matsunaga, Tadashi;Hiyama, Eiso
通讯作者：
Hiyama, Eiso
DOI：
--
发表时间：
2012
期刊：
Experience using extended criteria donors in first 100 cases of deceased donor liver transplantation in Japan. Transplant Proc
影响因子：
--
作者：
Furukawa H;Taniguchi M;Fujiyoshi M;Oota M;
通讯作者：
Oota M;