Evidence for FSH-dependent upregulation of SPATA2 (Spermatogenesis-associated protein 2)
Evidence for FSH-dependent upregulation of SPATA2 (Spermatogenesis-associated protein 2)
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DOI：
10.1006/bbrc.2001.4754
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发表时间：
2001-04-27
影响因子：
3.1
通讯作者：
Carlo, F
中科院分区：
文献类型：
--
作者：
Maurizio, O;Liliana, SM;Carlo, F
在这里，我们报告了一个新的cDNA命名为spata 2的克隆和表征。SPATA 2是PD 1的直系同源物，PD 1是一种人类睾丸蛋白，被认为在精子发生中发挥作用。spata 2序列揭示了编码511个氨基酸的蛋白质的开放阅读框架。用大鼠mRNA进行的北方印迹分析显示了2.2和4.0 kb的两种不同的转录物。用绿色荧光蛋白（GFP）标记重组SPATA 2并在HLtat转染的细胞中表达嵌合多肽表明SPATA 2位于细胞核中。RT-PCR分析显示，spata 2 mRNA在睾丸中表达，在大脑中表达程度较低，而骨骼肌和肾脏显示几乎不可见的信号。同样的分析表明，分离的支持细胞表达spata 2 mRNA。支持细胞治疗。FSH在体外以时间依赖性方式诱导Spata 2 mRNA稳态水平的显著变化。在发育中的睾丸spata 2转录本首次检测到产后10天，表达水平随着年龄的增长稳步上升。FSH刺激spata 2 mRNA表达及其发育表达的能力表明，该蛋白可能在调节精子发生中起作用，因此，根据基因命名委员会，我们建议将该蛋白（或基因）命名为SPATA 2（精子发生相关蛋白2）。(C)北京：科学出版社.
Here we report the cloning and characterization of a novel cDNA named spata 2. SPATA2 is the ortholog of PD1, a human testicular protein which has been suggested to play a role in spermatogenesis. The spata 2 sequence reveals an open reading frame encoding a protein of 511 amino acids. Northern blot analysis with rat mRNA demonstrated two distinct transcripts of 2.2 and 4.0 kb. Tagging recombinant SPATA2 with the green fluorescent protein (GFP) and expressing the chimeric polypeptide in HLtat transfected cells indicated that SPATA2 is located in the nucleus. RT-PCR analysis revealed that spata 2 mRNA is expressed in the testis and to a lesser extent in the brain while skeletal muscle and kidney showed a barely visible signal. The same analysis demonstrated that isolated Sertoli cells express spata 2 mRNA. Treating Sertoli cells with. FSH in vitro induced remarkable changes in the steady-state level of spata 2 mRNA in a time-dependent manner. In developing testis spata 2 transcripts were first detected 10 days post partum and expression levels increased steadily with age. The ability of FSH to stimulate spata 2 mRNA expression as well as its developmental expression suggests that this protein might play a role in regulating spermatogenesis and thus, according to the Gene Nomenclature Committee, we propose the name SPATA2 (Spermatogenesis associated protein 2) for this protein (or gene). (C) 2001 Academic Press.