A comparative study between the adsorption of IgY and IgG on latex particles
A comparative study between the adsorption of IgY and IgG on latex particles
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DOI：
10.1163/156856200743931
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发表时间：
2000-01-01
影响因子：
3.6
通讯作者：
Hidalgo-Alvarez, R
中科院分区：
文献类型：
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作者：
Dávalos-Pantoja, L;Ortega-Vinuesa, JL;Hidalgo-Alvarez, R
用卵黄抗体(IGY)代替来自哺乳动物的IGE可能在发展常规诊断免疫分析中具有几个优点，一方面，由于抗体是直接从鸡蛋中获得的，因此减少了动物的痛苦。另一方面，IyY的使用避免了类风湿因子的干扰。在许多免疫分析中，类风湿因子与Ige分子相互作用，导致假阳性结果。尽管有这些优点，卵黄抗体却很少使用。作为开发基于卵黄抗体-乳胶凝集的诊断试验的一部分，对卵黄抗体-乳胶复合物的某些特性进行了初步研究。在这项工作中，分析了蛋白质的吸附和解吸、等电点、电动迁移率和胶体稳定性。将结果与IGC获得的结果进行了比较。观察到了有趣的差异(主要是由于TGY和Ig G的分子结构的不同)，表明Ig Y是比Ig G更亲水的分子。此外，包被IgY的INTER颗粒的胶体分散体更稳定(在pn为8)，而不是由Ig G致敏的。
The use of egg yolk antibodies (IgY) instead of Ige from mammalian species may present several advantages in the development of routine diagnostic immunoassays, On the one hand, the animal suffering is reduced, as antibodies are obtained directly from the egg. On the other hand, the use of IyY avoids the rheumatoid factor interference. The rheumatoid factor interacts with Ige molecules in many immunoassays causing false positive results. Despite these advantages, IgY antibodies are scarcely used. As part of an aim to develop a diagnostic test based on IgY-latex agglutination, a preliminary study on some characteristics of the IgY-latrx complexes is carried out. In this work, protein adsorption and desorption, isoelectric point, electrokinetic mobility, and colloidal stability are analysed. Results are compared to those obtained by IgC. Interesting differences are observed (which mainly arise from the difference in molecular structure between TgY and IgG), suggesting that IgY is a more hydrophohic molecule than IgG. In addition, colloidal dispersions; of IgY-covered Inter particles are more stable (at pn 8) than those sensitized by IgG.