Concomitant translocation of Purα with its binding proteins (PurBPs) from nuclei to cytoplasm during neuronal development
Concomitant translocation of Purα with its binding proteins (PurBPs) from nuclei to cytoplasm during neuronal development
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DOI：
10.1016/j.neures.2004.09.009
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Kuo, CH
中科院分区：
文献类型：
--
作者：
Zeng, LH;Okamura, K;Kuo, CH
在P4-P10期小鼠脑核提取物中发现两种puralpha结合蛋白（purbp）。在P14时，它们在核提取物中显著减少，在S3部分中增加，表明它们在发育过程中发生了动态易位。Western blot分析还显示，在P7-P14期间，当神经元回路进行时，Puralpha与purbp同时发生易位。免疫细胞化学研究证实，大鼠E18海马神经元在7-14天后核Puralpha转移到细胞质中。这些结果表明，Puralpha与purbp从细胞核到细胞质的时空易位在神经元发育中起着至关重要的作用。(C) 2004爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Two Puralpha-binding proteins (PurBPs) were found in nuclear extract from mouse brain during P4-P10 by the overlay assay. At P14, they were decreased significantly in nuclear extract and increased in the S3 fraction, indicating their dynamic translocation during development. Western blot analysis also demonstrated concomitant translocation of Puralpha with the PurBPs during P7-P14, when neuronal circuit proceeds. Immunocytochemical study with cultured hippocampal neurons from rat E18 confirmed that nuclear Puralpha was translocated to cytoplasm after plating for 7-14 days. These results suggest that spatiotemporal translocation of Puralpha with the PurBPs from nuclei to cytoplasm has a crucial role in neuronal development. (C) 2004 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.