Template-Directed Synthesis of Novel, Nucleic Acid-Like Structures
Template-Directed Synthesis of Novel, Nucleic Acid-Like Structures
复制标题
新型核酸样结构的模板定向合成
DOI：
10.1126/science.228.4699.585
复制
发表时间：
1985
期刊：
影响因子：
56.9
通讯作者：
L. Orgel
中科院分区：
文献类型：
--
作者：
A. Schwartz;L. Orgel
在研究生命起源时，重要的是检查可能在原始地球上积累的底物混合物的反应。在益生元条件下，核苷二磷酸可能与标准核苷酸一起合成。由于这些原因，研究了这些二磷酸酯的活化衍生物（单独或与活化核苷酸混合）的模板引导反应。脱氧鸟苷 3',5'-二磷酸的活化衍生物在聚胞苷酸模板上有效缩合，得到寡核苷酸类似物，其中每个 3',5'-磷酸二酯键被焦磷酸键取代。即使没有模板，低聚物也会形成，但速度要慢得多。模板引导的缩合也用类似的脱氧腺苷衍生物在多尿苷酸上和用类似的无环鸟苷衍生物在多胞苷酸上发生。
In studying the origins of life, it is important to examine reactions of substrate mixtures that could plausibly have accumulated on the primitive earth. Nucleoside diphosphates would probably have been synthesized along with the standard nucleotides under prebiotic conditions. For these reasons, the template-directed reactions of activated derivatives of these diphosphates, alone or mixed with activated nucleotides, were investigated. An activated derivative of deoxyguanosine 3',5'-diphosphate condensed efficiently on a polycytidylate template to give oligonucleotide analogues in which each 3',5'-phosphodiester bond was replaced by a pyrophosphate linkage. Oligomers were formed even in the absence of a template, but much more slowly. Template-directed condensation occurred also with an analogous deoxyadenosine derivative on polyuridylic acid and with an analogous acycloguanosine derivative on polycytidylic acid.