Prevention of intestinal adhesion and regeneration of abdominal wall tissue with meshes containing an electrostatically spun acellular dermal matrix (ADM)/silk fibroin (SF) fiber composite polypropylene mesh.
Prevention of intestinal adhesion and regeneration of abdominal wall tissue with meshes containing an electrostatically spun acellular dermal matrix (ADM)/silk fibroin (SF) fiber composite polypropylene mesh.
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DOI：
10.1016/j.jmbbm.2020.104087
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发表时间：
2020-09
影响因子：
3.9
通讯作者：
Dongchao Yang;Zhicheng Song;Yongjia Lin;Wenpei Dong;Shao-ju Fu;Jian-jun Yang;Pei-hua Zhang;Yan Gu
中科院分区：
文献类型：
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作者：
Dongchao Yang;Zhicheng Song;Yongjia Lin;Wenpei Dong;Shao-ju Fu;Jian-jun Yang;Pei-hua Zhang;Yan Gu
腹壁缺损的修复往往需要使用聚丙烯（PP）作为主要材料。 PP网片植入体内后，与肠道接触会导致肠道与网片粘连，导致肠瘘等严重并发症。在这项研究中，我们使用静电纺丝技术在聚丙烯网的一侧涂覆一层由无细胞真皮基质 (ADM)/丝素蛋白 (SF) 混合材料制成的静电纺丝隔离层。这些网片用于修复模型大鼠腹壁缺损，并与聚己内酯（PCL）复合聚丙烯网片和PP网片进行比较。结果表明，ADM/SF-PP网的粘附分数和面积均小于PCL-PP和PP网。免疫组织化学评估表明，ADM/SF网片可以有效减轻网片与腹部器官接触面的炎症反应。腹侧再生的组织富含新血管。此外，ADM/SF网可以有效降低炎症相关因子IL-6和TNF-α的表达水平。 ADM/SF-PP 网片介导的修复后组织再生相关因子（如 VEGF 和 PAX-7）的表达水平也高于 PCL-PP 网片和 PP 网片修复后的表达水平。因此，ADM/SF-PP网片可以有效减轻网片与腹部器官接触面的炎症反应，快速促进腹部表面组织再生，预防和减少腹部粘连，支持腹壁修复。
The repair of abdominal wall defects often requires the use of polypropylene (PP) as the main material. After a PP mesh is implanted in the body, contact with the intestine can cause adhesions between the intestine and the mesh, leading to serious complications such as intestinal fistula. In this study, we used electrostatic spinning technology to coat one side of PP meshes with an electrostatically spun isolating layer of acellular dermal matrix (ADM)/silk fibroin (SF) hybrid material. These meshes were used to repair abdominal wall defects in model rats and were compared with polycaprolactone (PCL) composite polypropylene meshes and PP meshes. The results showed that the adhesion score and area of ADM/SF-PP meshes were smaller than those of PCL-PP and PP meshes. Immunohistochemical assessment revealed that the ADM/SF meshes could effectively reduce the inflammatory response at the contact surface between the meshes and abdominal organs. The tissues regenerated on the abdominal side were rich in new blood vessels. Furthermore, the ADM/SF meshes could effectively reduce the expression levels of the inflammation-related factors IL-6 and TNF-α. The expression levels of tissue regeneration-related factors, such as VEGF and PAX-7, were also higher after ADM/SF-PP mesh-mediated repair than after PCL-PP mesh and PP mesh repair. Thus, ADM/SF-PP meshes can effectively reduce the inflammatory response at the contact surface between the meshes and abdominal organs and quickly promote regeneration of abdominal surface tissue to prevent and reduce abdominal adhesion and support restoration of the abdominal wall.