Fabrication of bimodal porous PLGA scaffolds by supercritical CO2 foaming/particle leaching technique
Fabrication of bimodal porous PLGA scaffolds by supercritical CO2 foaming/particle leaching technique
复制标题
超临界CO2发泡/颗粒浸出技术制备双峰多孔PLGA支架
DOI：
10.1002/app.43644
复制
发表时间：
2016
影响因子：
3
通讯作者：
Shan-Jing Yao
中科院分区：
文献类型：
--
作者：
Xin Xin;Qian-Qian Liu;Chuan-Xin Chen;Yi-Xin Guan;Shan-Jing Yao
特殊的孔结构是组织工程支架材料的重要组成部分。本文采用超临界CO2（scCO 2）发泡和颗粒沥滤相结合的方法制备了具有大孔和微孔的双峰孔结构的聚乳酸-羟基乙酸共聚物（PLGA）支架，以促进营养物质的转移和细胞的粘附。三种不同尺度的NaCl颗粒（即，100-250、<75、<10 μm）分别用作致孔剂。特别地，当使用NaCl亚微米颗粒（<10 μm）作为致孔剂时，异相成核发生以改进scCO 2发泡/颗粒浸出过程。对PLGA支架的观察表明，在大孔（孔径约100-300 μm）的细胞壁中形成微孔（孔径<10 μm），呈现双峰孔结构。在不同NaCl质量分数下，3种NaCl致孔剂制备的PLGA支架孔隙率为68.4 ± 1.4 ~ 88.7 ± 0.4%。扫描电镜、能谱分析和体外细胞毒性试验结果表明，PLGA支架结构均匀，细胞增殖良好，无毒性。这种新的scCO 2发泡/颗粒沥滤方法由于其能够制造具有精确孔结构和高孔隙率的支架，在组织工程中是有前途的。© 2016 Wiley Periodicals，Inc. J.应用聚合物Sci. 2016，133，43644。
Specific pore structure is a vital essential for scaffolds applied in tissue engineering. In this article, poly(lactide-co-glycolide) (PLGA) scaffolds with a bimodal pore structure including macropores and micropores to facilitate nutrient transfer and cell adhesion were fabricated by combining supercritical CO2 (scCO2) foaming with particle leaching technique. Three kinds of NaCl particles with different scales (i.e., 100–250, <75, <10 μm) were used as porogens, respectively. In particular, heterogeneous nucleation occurred to modify scCO2 foaming/particle leaching process when NaCl submicron particles (<10 μm) were used as porogens. The observation of PLGA scaffolds gave a formation of micropores (pore size <10 μm) in the cellular walls of macropores (pore size around 100–300 μm) to present a bimodal pore structure. With different mass fractions of NaCl introduced, the porosity of PLGA scaffolds ranged from 68.4 ± 1.4 to 88.7 ± 0.4% for three NaCl porogens. The results of SEM, EDS, and in vitro cytotoxicity test of PLGA scaffolds showed that they had uniform structures and were compatible for cell proliferation with no toxicity. This novel scCO2 foaming/particle leaching method was promising in tissue engineering due to its ability to fabricate scaffolds with precise pore structure and high porosity. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43644.