Structural analysis of glycogen-like polysaccharides having macrophage-activating activity in extracts of Lentinula edodes mycelia
Structural analysis of glycogen-like polysaccharides having macrophage-activating activity in extracts of Lentinula edodes mycelia
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DOI：
10.1007/s11418-009-0357-1
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发表时间：
2010-01-01
影响因子：
3.3
通讯作者：
Tamesada, Makoto
中科院分区：
文献类型：
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作者：
Kojima, Hiroaki;Akaki, Junji;Tamesada, Makoto
香菇菌丝体（L.E.M.）在包含甘蔗渣和米糠的培养基中培养后用热水获得。据报道，L.E.M.显示出生理作用，例如抗癌活性和免疫调节活性，并且这些作用涉及巨噬细胞活化。我们检测了L.E.M.中的活性成分通过使用阴离子交换载体和凝胶色谱进行分级分离，并确定它们是否刺激RAW 264.7细胞中细胞因子肿瘤坏死因子-α（TNF-α）的产生。我们获得了2个多糖级分，IA-a和IA-b，其有效地刺激细胞因子的产生。我们还证实了IA-a和IA-b显著刺激RAW 264.7细胞的吞噬作用。为了确定这些级分的结构，我们进行了糖组分分析，甲基化分析，FT-IR，C-13-NMR分析和分子量分析。结果表明，IA-a的主要组分是1，4-α-葡聚糖，其表现出1，6-分支并具有约250，000的分子量。这种分支结构表明IA-a具有糖原样结构。此外，IA-b的主要成分似乎是阿拉伯聚糖样多糖，主要由阿拉伯糖和木糖组成，分子量约为8，500。
An extract of Lentinula edodes mycelia (L.E.M.) was obtained with hot water after culturing in medium comprising bagasse and rice bran. It has been reported that L.E.M. exhibits physiological effects such as anticancer activity and immunoregulatory activity, and that such effects involve macrophage activation. We examined the active components in L.E.M. by fractionation using an anion exchange carrier and gel chromatography, and determined whether they stimulate production of the cytokine tumor necrosis factor-alpha (TNF-alpha) in RAW264.7 cells. We obtained 2 polysaccharide fractions, IA-a and IA-b, which potently stimulated cytokine production. We also confirmed that IA-a and IA-b significantly stimulate phagocytosis in RAW264.7 cells. To determine the structures of these fractions, we performed sugar component analysis, methylation analysis, FT-IR, C-13-NMR analysis, and molecular weight analysis. The results showed that the major component of IA-a is a 1,4-alpha-glucan exhibiting 1,6-branching and having a molecular weight of approximately 250,000. This branching structure suggests that IA-a has a glycogen-like structure. In addition, the major component of IA-b appears to be an arabinoxylan-like polysaccharide, mainly consisting of arabinose and xylose, with a molecular weight of approximately 8,500.