Regulation of copy number and stability of phage λ derived pTCλ1 plasmid in the light of the dimer/multimer catastrophe hypothesis
Regulation of copy number and stability of phage λ derived pTCλ1 plasmid in the light of the dimer/multimer catastrophe hypothesis
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DOI：
10.1111/j.1574-6968.1999.tb13702.x
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发表时间：
1999-07-15
影响因子：
2.1
通讯作者：
Wegrzyn, G
中科院分区：
文献类型：
--
作者：
Herman-Antosiewicz, A;Wegrzyn, G
二聚体突变假说先前已被提出来解释不稳定的多拷贝质粒的分区是随机的，相反的低拷贝数质粒的稳定维持和积极分区。到目前为止，这一假设已经使用多拷贝ColE 1质粒进行了研究。然而，为了更详细地检验二聚体/多聚体突变假说，应该使用可以维持在低或高拷贝数并且仍然具有相同的复制调节机制的质粒。在这里，我们使用了一种修饰的λ质粒，pTC λ 1。该质粒的优点在于，它可以根据刺激质粒复制起始的诱导剂的浓度而保持不同的拷贝数。用该质粒在重组熟练和缺陷细胞中获得的结果通常支持二聚体/多聚体突变假说，但也表明模型中的一些修改。(C)1999年由Elsevier Science B. V.出版，版权所有。
The dimer catastrophe hypothesis has been proposed previously to explain instability of multicopy plasmids whose partitioning is random, contrary to low copy number plasmids which are stably maintained and actively partitioned. Until now, this hypothesis has been investigated using multicopy ColE1 plasmids. However, for more detailed testing of the dimer/multimer catastrophe hypothesis, one should use a plasmid which can be maintained at either low or high copy number and still possesses the same mechanism of replication regulation. Here we used a modified lambda plasmid, pTC lambda 1. The advantage of this plasmid is that it can be maintained at different copy numbers depending on the concentration of an inducer which stimulates the initiation of plasmid replication. Results obtained with this plasmid in recombination proficient and deficient cells generally support the dimer/multimer catastrophe hypothesis, but also suggest some modification in the model. (C) 1999 Published by Elsevier Science B.V. All rights reserved.