Novel bright field molecular morphology methods for detection of HER2 gene amplification
Novel bright field molecular morphology methods for detection of HER2 gene amplification
复制标题
用于检测 HER2 基因扩增的新型明场分子形态学方法
DOI：
10.1007/s10735-004-2191-9
复制
发表时间：
2004
影响因子：
3.2
通讯作者：
J. Hainfeld
中科院分区：
文献类型：
--
作者：
R. Tubbs;J. Pettay;D. Hicks;M. Skacel;R. Powell;T. Grogan;J. Hainfeld
分析HER2基因的扩增和过表达是确定浸润性乳腺癌预后和管理的关键组成部分。HER2基因扩增和过度表达的临床实验室检测因不可接受的高假阳性免疫组化（IHC）结果率、IHC评分“2+”类别的重现性差以及诊断病理学界不愿接受荧光原位杂交（FISH）替代检测而变得复杂。新的显色原位杂交（CISH）检测已经开发出，利用明场显微镜和传统的光学显微镜的解释，但第一代CISH系统的分析灵敏度一直是有问题的。已经开发了基于聚合Ig检测化学、自动荧光照相术或酶金相学的新型第二代原位杂交检测方法，其常规检测正常细胞中的内源性HER2信号（在载玻片杂交对照上）和HER2基因组特征的非扩增和扩增模式中的HER2信号。通过结合聚合过氧化物酶标记抗体的强度和用于基因扩增的金相学，以及通过IHC在同一载玻片上检测HER2编码蛋白的表达，可以在癌的每个显微镜视野中逐个细胞地同时评估HER2基因扩增和蛋白过度表达。
Profiling the amplification and over-expression of the HER2 gene is a key component for defining the prognosis and management of invasive breast carcinoma. Clinical laboratory testing for HER2 gene amplification and over expression has been complicated by an unacceptably high rate of false positive immunohistochemistry (IHC) results, poor reproducibility for the '2+' category of IHC scoring, and reluctant acceptance of alternative testing by fluorescence in situ hybridization (FISH) by the diagnostic pathology community. Novel chromogenic in situ hybridization (CISH) assays have been developed that utilize bright field microscopy and a conventional light microscope for interpretation, but the analytical sensitivity of first generation CISH systems has been problematic. Novel second generation in situ hybridization detection methods based upon polymerized lg detection chemistry, autometallography or enzyme metallography, have been developed that routinely detect endogenous HER2 signals in normal cells (on slide hybridization control) and HER2 signals in both non-amplified and amplified patterns of HER2 genomic signatures. By combining the strength of polymerized peroxidase-labeled antibodies and metallography for gene amplification, with the detection of expression of HER2 encoded protein by IHC on the same slide, both HER2 gene amplification and protein over-expression can be simultaneously evaluated on a cell-by-cell basis in each microscopic field of carcinoma.
影响因子：
45.3
作者：
Press, MF;Slamon, DJ;Bernstein, L
通讯作者：
Bernstein, L
金促进原位杂交：荧光原位杂交的明场自金相替代方法，用于检测 Her-2/neu 基因扩增。
DOI：
10.1016/s0002-9440(10)61106-6
发表时间：
2002
期刊：
The American journal of pathology
影响因子：
--
作者：
Tubbs,Raymond;Pettay,James;Skacel,Marek;Powell,Richard;Stoler,Mark;Roche,Patrick;Hainfeld,James
通讯作者：
Hainfeld,James
档案组织样本中 HER-2/neu 抗体的敏感性：癌基因表达免疫组织化学研究中潜在的错误来源。
影响因子：
11.2
作者：
Press,MF;Hung,G;Godolphin,W;Slamon,DJ
通讯作者：
Slamon,DJ