GLUCOSE-METABOLISM IN ISOLATED BROWN ADIPOCYTES UNDER BETA-ADRENERGIC STIMULATION - QUANTITATIVE CONTRIBUTION OF GLUCOSE TO TOTAL THERMOGENESIS
GLUCOSE-METABOLISM IN ISOLATED BROWN ADIPOCYTES UNDER BETA-ADRENERGIC STIMULATION - QUANTITATIVE CONTRIBUTION OF GLUCOSE TO TOTAL THERMOGENESIS
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DOI：
10.1042/bj2450789
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发表时间：
1987-08-01
影响因子：
4.1
通讯作者：
MEIER, MK
中科院分区：
文献类型：
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作者：
ISLER, D;HILL, HP;MEIER, MK
为了定量棕色脂肪组织作为葡萄糖氧化的靶器官的潜力，在存在和不存在胰岛素的情况下，通过使用β-葡萄糖代谢测定分离的大鼠棕色脂肪细胞中的O2消耗和葡萄糖代谢。激动剂异丙肾上腺素或Ro 16-8714刺激产热。用异丙肾上腺素（20 nM）和Ro 16-8714（20 μ M）分别最大地刺激基础代谢速率（278 μ mol O2/h/g脂质）至1633和1024 μ mol O2/h/g，而胰岛素对O2消耗没有影响。总葡萄糖摄取，来自[U-14 C]葡萄糖掺入CO2和总脂质和乳酸释放的总和，与胰岛素增强。异丙肾上腺素和Ro 16-8714对胰岛素诱导的葡萄糖摄取没有影响，但促进葡萄糖氧化，同时抑制胰岛素依赖性脂肪生成和乳酸产生。在Ro 16-8714和胰岛素的联合作用下获得葡萄糖氧化的最大值，其对应于每g脂质165 μ mol O2/h的当量。这表明葡萄糖是分离的棕色脂肪细胞的次要底物，最多可促进16%的产热。
To quantify the potential of brown adipose tissue as a target organ for glucose oxidation, O2 consumption and glucose metabolism in isolated rat brown adipocytes were measured in the presence and absence of insulin, by using the .beta.-agonists isoprenaline or Ro 16-8714 to stimulate thermogenesis. Basal metabolic rate (278 .mu.mol of O2/h per g of lipid) was maximally stimulated with isoprenaline (20 nM) and Ro 16-8714 (20 .mu.M) to 1633 and 1024 .mu.mol of O2/h per g respectively, whereas insulin had no effect on O2 consumption. Total glucose uptake, derived from the sum of [U-14C]glucose incorporation into CO2 and total lipids and lactate release, was enhanced with insulin. Isoprenaline and Ro 16-8714 had no effect on insulin-induced glucose uptake, but promoted glucose oxidation while inhibiting insulin-dependent lipogenesis and lactate production. A maximal value for glucose oxidation was obtained under the combined action of Ro 16-8714 and insulin, which corresponded to an equivalent of 165 .mu.mol of O2/h per g of lipid. This makes it clear that glucose is a minor substrate for isolated brown adipocytes, fuelling thermogenesis by a maximum of 16%.