Cancer-derived Exosomes Activate Immune Surveillance and Suppress Peritoneal Metastasis of Murine Colonic Cancer
Cancer-derived Exosomes Activate Immune Surveillance and Suppress Peritoneal Metastasis of Murine Colonic Cancer
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DOI：
10.21873/anticanres.14890
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发表时间：
2021-03
影响因子：
2
通讯作者：
Aya Tokuda;Toru Miyake;Daiki Yasukawa;Daiji Ikuta;K. Mukaisho;S. Murata;Tomoharu Shimizu;M. Tani
中科院分区：
文献类型：
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作者：
Aya Tokuda;Toru Miyake;Daiki Yasukawa;Daiji Ikuta;K. Mukaisho;S. Murata;Tomoharu Shimizu;M. Tani
背景：与胃癌相比，结肠癌的腹膜转移率较低，但其原因尚不清楚。在这项研究中，使用CT26小鼠结肠癌细胞系建立的腹膜扩散模型被用来分析癌症来源的外切体的生理作用。材料和方法：采用超速离心法从CT26细胞培养上清液中收集外切小体。比较了两种结肠癌小鼠模型在给药外切体或磷酸盐缓冲盐水前的腹膜转移数目。结果：与磷酸盐缓冲液相比，癌症来源的外切体抑制了腹膜扩散。注射外切体后，小鼠腹腔巨噬细胞数量增加，诱导型一氧化氮合酶表达增加。此外，癌症来源的外切体增加了激活的自然杀伤细胞和干扰素-γ的表达。结论：结肠癌来源的外切体可能通过免疫机制抑制腹膜转移。
Background: Colonic cancer is associated with a low incidence of peritoneal metastasis compared with gastric cancer; however, the reason for this remains unclear. In this study, a model of peritoneal dissemination using the CT26 murine colon cancer cell line was used to analyze the physiological roles of cancer-derived exosomes. Materials and Methods: Exosomes were collected from the supernatant of CT26 cell culture by ultracentrifugation. The number of peritoneal disseminations in two mouse models of colonic cancer pre-administered exosomes or phosphate-buffered saline were compared. Results: Cancer-derived exosomes suppressed peritoneal dissemination compared to phosphate-buffered saline. After administration of exosomes, the number of intraperitoneal macrophages and the expression of inducible nitric oxide synthase increased. Furthermore, cancer-derived exosomes increased activated natural killer cells and interferon-γ expression. Conclusion: Tumor-derived exosomes from colonic cancer may suppress peritoneal metastasis via an immunological mechanism.