Serotonin metabolism in neonatal rat brain during asphyxia and recovery.
Serotonin metabolism in neonatal rat brain during asphyxia and recovery.
复制标题
窒息和恢复期间新生大鼠大脑中的血清素代谢。
DOI：
--
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
P. Lundborg
中科院分区：
文献类型：
--
作者：
T. Hedner;P. Lundborg
新生大鼠暴露于20或30分钟的全部或部分缺氧。在窒息和随后的恢复色氨酸和5-羟色胺（5-HT，血清素）的内源性水平进行了测量。色氨酸羟化酶是5-羟色胺合成途径中的第一个限速酶，通过测定用NSD 1015抑制芳香族L-氨基酸脱羧酶后5-羟色氨酸（5-HTP）的积累，在体内研究了色氨酸羟化酶的活性。在窒息期间，色氨酸羟化酶的活性在整个大脑和研究的各个区域都有所下降。色氨酸、5-羟色胺和5-羟色胺水平在窒息恢复30分钟后均升高。全脑5-HTP和5-HT水平在缺氧后2 h恢复正常，而色氨酸水平在缺氧后6 h恢复正常较慢。在局部脑研究中，窒息后纹状体和中脑的5-HTP水平迅速恢复到对照水平，但脑干和半球区域的5-HTP水平没有恢复。全脑5-HTP和5-HT水平在窒息后24 ~ 48 h与对照组无差异。虽然新生儿神经系统对窒息表现出很大的抵抗力，但神经递质5-HT的代谢在短暂的窒息和随后的恢复期间已经受到影响。由于5-HT具有重要的神经递质功能，这可能与人类窒息后的神经系统后遗症有关。
Neonatal rats were exposed to 20 or 30 min of total or partial oxygen deprivation. During asphyxia and subsequent recovery the endogenous levels of tryptophan and 5-hydroxytryptamine (5-HT, serotonin) were measured. The activity of tryptophan hydroxylase, the first and rate limiting enzyme in the 5-HT synthesis pathway, was studied in vivo by measuring the accumulation of 5-hydroxytryptophan (5-HTP) after inhibition of aromatic L-amino acid decarboxylase with NSD 1015. During asphyxia there was a decrease in tryptophan hydroxylase activity in the whole brain and various regions studied. The levels of tryptophan, 5-HTP and 5-HT all increased after 30 min of recovery from asphyxia. In the whole brain, 5-HTP and 5-HT levels were normal 2 h after anoxia while tryptophan levels normalized more slowly to reach control values after 6 h. In the regional brain study, the 5-HTP levels returned quickly to control levels after asphyxia in the striatum and midbrain but not in the brainstem and hemispheres regions. The whole brain 5-HTP and 5-HT levels did not differ from controls 24 to 48 h after the asphyxia. Although the neonatal nervous system exhibits a great resistance to asphyxia, the metabolism of the neurotransmitter 5-HT is affected already during a short period of asphyxia and subsequent recovery. As 5-HT is ascribed important neurotransmitter functions, this might be relevant to the neurological sequelae of human asphyxia neonatorum.