Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids
Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids
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DOI：
10.2135/cropsci1997.0011183x003700030027x
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发表时间：
1997-05-01
期刊：
影响因子：
2.3
通讯作者：
Hamilton, RI
中科院分区：
文献类型：
--
作者：
Hodges, DM;Andrews, CJ;Hamilton, RI
对低温敏感的物种通常比耐冷的物种具有更低的抗氧化能力。本研究旨在确定抗氧化能力和碳水化合物浓度在玉米（Zea mays L.）低温敏感性筛选技术中是否有用。在(i)恒定25℃的控制条件下，或（ii）控制条件下加上收获前1天11℃的短期冷激，在第三叶期收获12个对寒冷有不同敏感性的玉米杂交种的叶片。对过氧化氢酶（CAT; EC 1.11.1.6）、单脱氢抗坏血酸还原酶（MDHAR; EC 1.6.5.4）和抗坏血酸过氧化物酶（ASPX; EC 1.11.1.11）活性进行了评估。碳水化合物水平作为低温胁迫的一般代谢指标。在对低温敏感的杂种中，CAT、MDHAR和ASPX的冷控比最低，碳水化合物浓度最高。杂种对有毒氧化合物解毒的抗氧化能力的差异可能对不同的冷敏感性很重要。敏感杂交种低温处理后碳水化合物的冷控比较高，说明低温处理对其同化物利用率的影响大于耐冷杂交种。因此，CAT、MDHAR和ASPX活性和碳水化合物浓度可以作为玉米冷敏感性筛选技术中低温胁迫的优良指标。
Chilling sensitive species often have lower antioxidant capacities than do tolerant species. This study was conducted to determine if antioxidant capacities and carbohydrate concentrations would be useful in a proposed screening technique for chilling sensitivity in maize (Zea mays L.). Leaves of 12 maize hybrids exhibiting differential sensitivity to chilling were harvested at the third-leaf stage under either (i) a constant 25 degrees C control regime or (ii) the control regime plus a short-term chilling shock of 11 degrees C for 1 d prior to harvesting. Catalase (CAT; EC 1.11.1.6), monodehydroascorbate reductase (MDHAR; EC 1.6.5.4), and ascorbate peroxidase (ASPX; EC 1.11.1.11) activities were assessed. Carbohydrate levels were determined as general metabolic indicators of chilling stress. The chill/control ratios of CAT, MDHAR, and ASPX were lowest and concentrations of carbohydrates highest in the most chilling sensitive hybrids. Differences between hybrids in antioxidant capacities to detoxify toxic oxygen compounds may be important in differential chilling sensitivity. The higher chill/control ratios of carbohydrates in the sensitive hybrids after chilling indicated that their rate of assimilate utilization was reduced by the chilling treatment more so than those of the chilling tolerant hybrids. Activities of CAT, MDHAR, and ASPX and concentrations of carbohydrates would thus make excellent indicators of chilling stress in a screening technique for chilling sensitivity of maize.